FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914185
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA74150.8111049732822131No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42640.4664263797808978No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT37410.4092169528049574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33400.36535274588841427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30160.32991134179624476No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT29190.31930079797852734No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28540.31219063975016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28330.30989351170714896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28180.30825270596214116No Hit
GTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTCAGGAG27610.3020176441311113No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25670.2807965564956765No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC24920.27259252777063725No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA24380.26668562708860893No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24340.2662480788899402No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA23230.25410611637688213No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22880.2502775696385305No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22240.24327679845983033No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACGTTCAGGGAGA22050.24119844451615371No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21470.23485399563545672No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG19690.2153831007946969No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19610.2145080043973594No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG19310.2112263929073437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18730.2048819440266467No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18200.19908443039428564No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18200.19908443039428564No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18190.1989750433446184No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17890.19569343185460272No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17460.1909897887189136No Hit
GGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGCCGG17150.18759879017923067No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG17110.18716124198056192No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGA16890.18475472688788375No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16810.18387963049054623No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16480.18026985785152896No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA16450.1799416967025274No Hit
ATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA16430.179722922603193No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16230.1775351816098492No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCT16060.17567560176550698No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16000.17501927946750384No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGA15840.17326908667282878No Hit
AGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTC15260.1669246377921318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15240.1667058636927974No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC15080.16495567089812238No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14830.16222099465644263No Hit
GTGTATTACTGTGCGAGACGGGGGTATTGTGGTGGAGACTGCTGGGATGC14800.16189283350744105No Hit
GTCTCTGGTGGCTCCATCAGAAGTGCTAATTATTACTGGGGCTGGATCCG14740.1612365112094379No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14540.15904877021609412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14310.15653286807374875No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14230.15565777167641123No Hit
CTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGAT14190.15522022347774247No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14020.15336064363340024No Hit
GTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCT13890.1519386119877268No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCC13700.14986025804405015No Hit
GGCCAAAAGTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCA13600.14876638754737825No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT13390.1464692595043673No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACAC13260.1450472278586938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13140.14373458326268754No Hit
GTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATG13080.1430782609646844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12950.14165622931901092No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12840.14045297177267185No Hit
GTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATA12780.13979664947466872No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGACAATCAGTGCCG12590.13771829553099207No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12430.13596810273631704No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12230.13378036174297325No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11930.13049875025295754No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11890.13006120205428878No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11820.12929549270661847No Hit
GATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGC11770.1287485574582825No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA11640.12732652581260903No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11480.125576333017934No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11400.12470123662059648No Hit
GGTCAGGGACAGATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGAT11340.12404491432259335No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11230.12284165677625426No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11220.12273226972658707No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11160.12207594742858392No Hit
ATTCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGATAGTGGAGT11120.12163839922991516No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11090.1213102380809136No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGAAGTGCTAATTATTACTGGG11090.1213102380809136No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11050.12087268988224484No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10930.11956004528623856No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10720.11726291724322759No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10700.1170441431438932No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10690.11693475609422602No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10530.11518456329955096No Hit
GACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGC10470.11452824100154782No Hit
AGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTC10430.11409069280287906No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10320.11288743525653998No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10320.11288743525653998No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTC10290.11255927410753841No Hit
GCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTC10260.11223111295853684No Hit
GATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAAT10230.11190295180953527No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10220.11179356475986808No Hit
GTAGTAGGTGGTCCCACTATAATAGATTTTCCCTATCCACTGCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC10210.1116841777102009No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10160.11113724246186495No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10120.11069969426319617No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACGTTCAGGGA10100.1104809201638618No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGT10080.11026214606452742No Hit
GTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA10060.11004337196519304No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10050.10993398491552585No Hit
CTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTAT10030.10971521081619147No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG9970.10905888851818833No Hit
GATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTT9970.10905888851818833No Hit
ATGTGGCACTCCAGAGAATTGGTTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTA9610.10512095473016948No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAA9600.1050115676805023No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9540.10435524538249916No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9370.10249566553815694No Hit
CTCCATATCCGTGGACACGTCCAAGAATCAGTTCTCCCTGAACGTGAGGT9350.10227689143882256No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9330.10205811733948818No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9250.10118302094215066No Hit
GGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAG9220.1008548597931491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAG200.007136146552.15321448
TGGTATC1850.048.420612
GTGGTAT1950.045.960121
GTATCAA13400.043.473481
GGTATCA4950.039.6928371
TATCAAC15800.036.851762
CACGGAT7850.035.18737870
TCAACGC16650.034.7635154
CAACGCA16650.034.5565875
TAGAGTA400.002613423934.4531255
CTTAGAC400.002613423934.4531253
ATCAACG16900.034.249263
AACGCAG16950.034.148236
CTGTTTA750.00335557732.73729770
AGAGTAC18150.032.27014511
CGCAGAG18600.031.4894168
CAGAGTA18650.031.40499510
AAGGTTA450.004634397630.65014627
TTATCTC450.004653043630.6250024
TACACAT450.004653043630.6250025
Produced by FastQC (version 0.11.5)