FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914185
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA69890.7645060901239903No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT48460.5300896426872023No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41460.45351870792016935No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30840.33734966117361365No Hit
GTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTCAGGAG27380.29950174198876595No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26800.29315729310806893No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26780.2929385190087346No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26640.2914071003133939No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT25130.2748896558136482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24940.2728113018699716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23480.2568407926185619No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23180.2535591811285462No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21860.23912009057247713No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21600.2362760272811302No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21250.23244748054277853No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA21190.2317911582447754No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACGTTCAGGGAGA20780.2273062892084206No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA20420.2233683554204018No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20410.2232589683707346No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20310.22216509787406272No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC19430.21253903750335No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19270.21078884470867496No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19230.21035129651000617No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18520.2025848159836357No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA17080.18683308083156036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16700.18267637294420713No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16680.18245759884487275No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16640.182020050646204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16080.17589437586484136No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15960.17458173126883508No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA15870.1735972478218304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15820.1730503125734944No Hit
GTCTCTGGTGGCTCCATCAGAAGTGCTAATTATTACTGGGGCTGGATCCG15470.16922176583514278No Hit
GGCCAAAAGTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCA15230.1665964766431302No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14820.16211160760677545No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14640.16014264071276602No Hit
GTCCCTGACCCGCTGCCACTGAACCGATGTGGCACTCCAGAGAATTGGTT14560.1592675443154285No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG14420.15773612562008785No Hit
CCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTG14150.15478267527907372No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14140.1546732882294065No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14090.15412635298107058No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC14050.15368880478240182No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGA13680.14964148394471577No Hit
GGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGCCGG13560.1483288393487095No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGA13460.1472349688520376No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13420.14679742065336884No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCT13270.14515661490836101No Hit
GATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTG13170.1440627444116891No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13020.14242193866668126No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12980.1419843904680125No Hit
GTGTATTACTGTGCGAGACGGGGGTATTGTGGTGGAGACTGCTGGGATGC12980.1419843904680125No Hit
GTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTGGCACTCCA12900.141109294070675No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12870.1407811329216734No Hit
GTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATA12800.1400154235740031No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT12770.1396872624250015No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12690.13881216602766397No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12260.13410852289197484No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12220.13367097469330608No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12070.1320301689482982No Hit
ATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA11950.13071752435229192No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11830.12940487975628565No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGT11700.1279828481106122No Hit
CTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGAT11690.127873461060945No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACAC11670.12765468696161061No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11660.1275452999119434No Hit
TCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTGGCACT11640.12732652581260903No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGAAGTGCTAATTATTACTGGG11410.12481062367026367No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11280.12338859202459021No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11260.12316981792525583No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11200.1225134956272527No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11140.12185717332924954No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10850.11868494888890105No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10850.11868494888890105No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGACAATCAGTGCCG10820.11835678773989948No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10750.11759107839222915No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10720.11726291724322759No Hit
GGTCAGGGACAGATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGAT10640.11638782084589004No Hit
AGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTC10570.11562211149821972No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG10520.11507517624988377No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10510.11496578920021658No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10510.11496578920021658No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10370.11343437050487593No Hit
GTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCT10190.11146540361086651No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCC10140.11091846836253057No Hit
GTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA10120.11069969426319617No Hit
ATTCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGATAGTGGAGT10030.10971521081619147No Hit
GGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAG10020.10960582376652428No Hit
CTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTAT10000.1093870496671899No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9930.10862134031951957No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9890.10818379212085082No Hit
GATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTT9860.10785563097184925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9830.10752746982284768No Hit
GTAGTAGGTGGTCCCACTATAATAGATTTTCCCTATCCACTGCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC9820.10741808277318049No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9810.1073086957235133No Hit
GATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAAT9800.10719930867384611No Hit
ATGTGGCACTCCAGAGAATTGGTTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTA9730.10643359932617578No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9720.1063242122765086No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9680.10588666407783984No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9630.10533972882950389No Hit
GTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATG9480.10369892308449602No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9470.10358953603482883No Hit
GACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGC9300.10172995619048661No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9290.10162056914081942No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9260.10129240799181785No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACGTTCAGGGA9160.10019853749514596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)