FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939202
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCAGTAGTATCCCACAC20360.21677977687441038No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCA17640.18781902082832022No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG16990.1808982519202472No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16340.17397748301217417No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGA15160.16141362560982622No Hit
GGATTACCTGTGGGACAAACAACGTTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC13770.1466138274833316No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGACCCCT13650.1453361470695335No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12970.13809595805801095No Hit
GTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGAC12670.13490175702351573No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG12580.13394349671316713No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGACAAACAACGTTGGAAGT12580.13394349671316713No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12260.13053634894303887No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12140.12925866852924078No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11920.1269162544372776No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11840.12606446749474554No Hit
GTCTCTGGTGCCTCCAGCAGGAGTTTCTACTGGAGCTGGATCCGGCAGCC11570.12318968656369982No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11110.11829191164414044No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10640.11328766335676457No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT10540.11222292967859948No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10450.11126466936825091No Hit
ACCCTACTGATGGTCAGGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGAT10210.10870930854065472No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10090.10743162812685662No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9990.10636689444869156No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC9920.105621580873976No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9810.10445037382799441No Hit
GTATATTACTGTGCGAGAGGGATTTTATCTAGTGACCTCCCGGGGAACTGGTTCGACCCCTGGGGCCAGGGAACC9610.10232090647166424No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9430.10040438585096709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG200.007378628551.7144624
TCGTATG506.705159E-848.5147840
GGTATCA6800.044.130541
GTATCAA16750.041.8032571
TATCAAC18350.038.3278852
ATCAACG19450.036.1602443
CAACGCA19950.035.253975
TCAACGC20100.034.9908834
CTCGTAT709.441119E-734.6348839
AACGCAG20500.034.476316
TGGTATC2300.031.478372
AGAGTAC22350.031.47166311
CAGAGTA22800.030.8505110
AGACGTA902.001907E-730.65376925
ACGCAGA23400.030.3509397
CGCAGAG23300.030.3332338
GTGGTAT2450.029.5652891
GCAGAGT24300.029.08659
ACGTAGA953.213063E-729.04195827
CGTATGC854.267835E-628.54574241
Produced by FastQC (version 0.11.5)