FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187635
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39430.33200436160941704No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32080.2701166604217626No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28950.24376176182076145No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGGCTGCTATCCCATGTT28880.24317235514278376No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28090.23652047977703586No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28000.23576267119106462No Hit
GTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA27560.232057829215205No Hit
AGCTGGTACAGTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGATACA26360.22195371473558795No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATTTCCTG26190.22052229851764218No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24770.20856576305009533No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC23840.20073507432839216No Hit
CGTCCATACCGTACATCCAGTTAGCGGTCGCACAATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCT23590.1986300504784719No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT23530.19812484475449105No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC23300.19618822281256446No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGG22090.18599990737895059No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22060.18574730451696017No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21880.18423168734501763No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21850.1839790844830272No Hit
ATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATG21430.18044264441516122No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20330.17118053947551226No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19920.1677283003616431No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19860.16722309463766225No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18730.15770838683602284No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTA18550.15619276966408027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18390.15484555440013137No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18300.15408774581416007No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18300.15408774581416007No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18230.1534983391361824No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18180.15307733436619836No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18010.15164591814825262No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT17950.15114071242427177No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17790.14979349716032284No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17360.14617285613846004No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17220.14499404278250475No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17060.1436468275185558No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16760.1411207988986515No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16650.14019458840468663No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16310.13733175596879513No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA16020.13488992830288768No Hit
GGACAGAAGGTCACCATTTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATCTATCCTGGTACCAGCAG16020.13488992830288768No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16000.13472152639489404No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15610.13143768918901852No Hit
GAATTATCCATCCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG15130.12739604339717167No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15090.12705923958118445No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG14870.12520681859325467No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14770.12436480905328656No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14590.12284919188134402No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14440.12158617757139188No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTA14360.12091256993941742No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14310.12049156516943337No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG14280.12023896230744294No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAGTAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG14180.11939695276747485No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14180.11939695276747485No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14150.11914434990548442No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13800.11619731651559612No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA13530.11392389075768228No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGGTCCAGCTGGTA13520.11383968980368547No Hit
GCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGTCACGGTGACTCTG13280.11181886690776205No Hit
GTGTATTATTGTGCGACCGCTAACTGGATGTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC13170.11089265641379717No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA12890.10853502970188651No Hit
CTGTAGATGTGTCCTCGGTCACGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTTCACCATCTTCAGGAT12770.10752461825392483No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12730.1071878144379376No Hit
CACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAG12720.10710361348394076No Hit
CATCTACAGACACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTATTGTGCGACCG12610.10617740298997588No Hit
GTATCAACGCAGAGTACGGGATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCAGAGCGCAGCACCTCA12540.10558799631199822No Hit
CCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGTCACGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTT12530.1055037953580014No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAGGCTTTGATCCTGAAGATGGTGAAACAATCTACG12460.10491438868002374No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12170.10247256101411628No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC12080.10171475242814502No Hit
ACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTATTGT11940.10053593907218969No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11910.10028333621019926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)