FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176718
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC52440.4456462805871925No Hit
TTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCA32870.2793362555854504No Hit
ACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTCA30340.25783577713606826No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29220.24831777877112443No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGAGG29020.2466181362059559No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26160.2223132475240457No Hit
CTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCC25880.21993374793280973No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24410.2074413750788209No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22600.1920596098640456No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22450.19078487794016918No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21010.17854745147095566No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19500.16571515010393315No Hit
CCCCTGAGGGCCGCCGATCATTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGATA19260.1636755790257309No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18830.16002134751061853No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTC17820.15143815255651735No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17640.1499084742478657No Hit
GTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17000.14446961803932634No Hit
GACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTC16990.1443846359110679No Hit
GCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATA16850.14319488611544992No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16820.14293993973067465No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16620.1412402971655061No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15430.13112742390275325No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15190.12908785282455099No Hit
GTCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGA14550.12364899661601164No Hit
ATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13780.11710537274011276No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCC13150.11175149865983185No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12960.11013683822292172No Hit
CCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12790.10869214204252844No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12790.10869214204252844No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGACCATCAGTGGGCTCCGGT12760.10843719565775319No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12690.10784232075994418No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGG12650.10750239224691048No Hit
CTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12450.10580274968174193No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12130.10308332157747226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)