FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176718
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC45920.39023793296269793No Hit
TTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCA32290.2744072921464616No Hit
CTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCC29340.24933756431022555No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC28170.23939465530398957No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26990.22936676416949517No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC24330.20676151805275353No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24100.20480692910280965No Hit
ACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTCA23630.20081276907466358No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21780.18509107534685454No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19730.16766973905387697No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19210.16325066838443875No Hit
GTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18680.1587466155867421No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTC18130.1540725985325286No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17660.15007843850438252No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG17490.14863374232398927No Hit
CCCCTGAGGGCCGCCGATCATTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGATA16900.14361979675674208No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16490.14013552949814653No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15730.13367688775050607No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGAGG15200.12917283495280943No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14960.12713326387460716No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14860.1262834425920229No Hit
ATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14820.12594351407898918No Hit
GCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATA14390.12228928256387682No Hit
CCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG14070.11956985445960713No Hit
GTCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGA13870.11787021189443861No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGACCATCAGTGGGCTCCGGT13600.11557569443146107No Hit
CTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13550.11515078379016892No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGG13450.11430096250758465No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13250.11260131994241611No Hit
GACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTC12880.1094569811968543No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12390.10529285691219137No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11970.10172360752533742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18150.043.6942371
TATCAAC20100.038.581552
GGTATCA7700.035.6687661
CAACGCA22500.034.310765
TCAACGC22500.034.310764
ATCAACG22550.034.2346843
AACGCAG23300.033.1327136
AGAGTAC24600.030.4222811
GTGGTAT3400.030.2922231
CGCAGAG25500.030.1486178
TGGTATC3450.029.8379842
CAGAGTA25550.029.55984110
GCAGAGT26050.029.2560429
ACGCAGA26550.028.950157
GTATTAG1404.947651E-1026.9745041
ACGTTGG16350.026.9607870
TTAACGG658.6681824E-426.53269635
AACAAGG23450.024.9702370
ATTACTC1101.2094133E-624.9532813
TAGTACA1101.2094133E-624.9532814
Produced by FastQC (version 0.11.5)