FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077325
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21540.1999396653748869No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTGCAGTAATAAT20270.18815120785278353No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTG19670.182581857842341No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGACTGGTCAGCTCCATGTAGGCTGTGCCGATGGACGTGTCCCTGGT17000.15779825029587172No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16280.15111503028334067No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG16030.1487944677789896No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15920.14777342027707516No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14800.13737730025758244No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT14430.1339428677511429No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12580.11677070521894507No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12550.11649223771842294No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12550.11649223771842294No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11910.11055159770728423No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11730.10888079270415149No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11380.10563200519806001No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11190.1038683776947532No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11000.10210475019144642No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT10910.10126934768988002No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGATTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC10900.10117652518970598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2650.041.6260641
GTATCAA16850.040.915831
GGTATCA7050.038.1387751
TGGTATC2650.037.7078632
TATCAAC19000.036.0893672
CAACGCA20850.033.0524565
ATCAACG20950.032.7302133
TCAACGC21350.032.1174
TCGTATG652.2649976E-531.96090340
AACGCAG21700.031.9165656
ATACACG4050.029.7778154
CTCGTAT703.7669975E-529.66691239
AGAGTAC23050.029.44930611
GTAATAC4100.029.4269621
GTCGTCA4250.028.37782116
CAGAGTA24000.028.28360610
ACGCAGA24600.028.154047
CGCAGAG24700.028.0400548
TAATACA4600.027.7155632
GCAGAGT25050.027.6482819
Produced by FastQC (version 0.11.5)