FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077325
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19960.1852737103473882No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTGCAGTAATAAT18560.1722785603230223No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTG17260.1602116353003968No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGACTGGTCAGCTCCATGTAGGCTGTGCCGATGGACGTGTCCCTGGT16990.15770542779569768No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG16260.1509293852829926No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15370.14266818276750284No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT14900.13830552525932288No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14260.13236488524818416No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13270.12317545773095398No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13060.1212261852272991No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12330.11445014271459401No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11960.11101571020815444No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11650.10813821270275914No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11250.10442531269579747No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGATTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC11020.1022903951917945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.040.7840921
GTATCAA17700.036.730821
GTGGTAT2700.034.3987051
TAATACA4400.034.382742
TATCAAC18950.034.1105842
GTAATAC4300.033.5987361
ATACACG4250.032.360224
TCAACGC20900.030.9280154
ATCAACG21000.030.617013
CAACGCA21300.030.5086295
AACGCAG21800.029.808896
TACACGG5150.028.0402935
CGCAGAG23700.027.575748
CAGAGTA23500.027.2325310
AGAGTAC23550.027.17471311
GTCGTCA5500.026.27054216
GCAGAGT25000.026.1490869
ACGCAGA25200.025.9283087
GTATAAG2800.025.799031
TGTCGTC5700.025.35229915
Produced by FastQC (version 0.11.5)