FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976817
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC64260.657850958777335No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT59740.6115782178238094No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC59170.6057429385442719No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTT49770.5095120170922497No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT48220.4936441523847353No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCA46390.47490983469779907No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA45890.46979116866311704No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45040.46108943640415756No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44750.458120610104042No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC41450.42433741427514055No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCAC38670.39587763112230845No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37480.38369520595976525No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37260.3814429929045051No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG36770.3764267001905167No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG36470.3733555005697075No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35250.3608659554450834No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG33230.34018654466496795No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32660.3343512653854304No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA31930.32687801297479463No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG31080.3181762807158352No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30670.31397897456739593No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATT28360.2903307374871649No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26040.2665801270862403No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC25990.2660682604827721No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG25950.26565876719999754No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG25880.2649421539551421No Hit
GTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGG25080.2567522882996508No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24500.25081463569941964No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT24360.2493814092097087No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23670.24231765008184747No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC23090.23637999748161634No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22140.22665453201572044No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21920.22440231896046037No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21760.2227643458293621No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21560.22071687941548926No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA21510.22020501281202107No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGT21350.21856703968092284No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21260.21764567979468008No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC21110.21611007998427545No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGG20970.2146768534945645No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC20920.2141649868910963No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC20810.21303888036346622No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA20460.20945581413918882No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20420.20904632085641423No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20300.2078178410080906No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTAC20240.2072036010839287No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAA20200.20679410780115415No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC18760.19205234962126988No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAG18710.1915404830178017No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG18540.1898001365660098No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18200.18631944366242603No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17970.18396485728647227No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17900.1832482440416168No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17690.18109840430705035No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA17290.17700347147930473No Hit
GACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACT17240.1764916048758365No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16990.1739322718584955No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG16430.16819936589965162No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16090.16471867299606785No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT16070.16451392635468057No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15880.16256883326150137No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15800.16174984669595227No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGGCCAGGCCC15220.1558121940957211No Hit
GCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC15190.15550507413364018No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15000.153559981040461No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC14910.15263862115421825No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG14660.1500792881368772No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14490.14833894168508535No Hit
ACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG13970.143015529009016No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC13900.14229891576416054No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13830.14158230251930504No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG13740.14066094263306228No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13660.13984195606751318No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAACTGGGACCAAGGT13630.13953483610543224No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG13450.1376921163329467No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13450.1376921163329467No Hit
AGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCC13330.136463636484623No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC13290.13605414320184847No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13220.13533752995699297No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA13090.13400667678797562No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12910.1321639570154901No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12880.1318568370534092No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA12860.13165209041202192No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG12570.12868326411190634No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12370.12663579769803351No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA12350.12643105105664623No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT12340.12632867773595258No Hit
CCTCATAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCAT12340.12632867773595258No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC12250.12540731784970982No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12160.12448595796346706No Hit
GTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACA12150.12438358464277341No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12050.12335985143583701No Hit
TAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTGTTGTCCT11990.12274561151167517No Hit
CCTTGACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGT11940.12223374490820697No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11870.12151713166335147No Hit
GGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGG11680.11957203857017232No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG11640.11916254528739774No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11410.116807958911444No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG11400.11670558559075037No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC11350.11619371898728217No Hit
TAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGC11270.11537473242173304No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11240.11506761245965212No Hit
AGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAG11200.11465811917687756No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG11160.11424862589410299No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG11040.1130201460457793No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC10920.11179166619745562No Hit
GAGTAACAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGT10880.11138217291468105No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10750.11005131974566372No Hit
GAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTGTTGTCCTCATAGATGACCAGCACAGGGGC10730.10984657310427644No Hit
CTCCAGAGCAGGTGATCCTGGCCGTTTGTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGG10720.10974419978358281No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG10710.10964182646288916No Hit
GCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATT10700.10953945314219553No Hit
GATTACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCC10670.10923233318011459No Hit
ACAGACAGCAGTGGTAATCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC10640.10892521321803367No Hit
AGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGC10450.1069801201248545No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG10430.10677537348346722No Hit
GCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGT10310.10554689363514354No Hit
GTCGTTTGTTGTCCTCATAGATGACCAGCACAGGGGCCTGGCCTGACTTC10240.10483028039028805No Hit
GCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCT10150.1039089205040453No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG10070.10308993393849616No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT10070.10308993393849616No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC9980.1021685740522534No Hit
TCTTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATC9940.10175908076947883No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC9930.10165670744878517No Hit
ACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCC9860.1009400942039297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGT252.5549033E-455.2527538
TTAGTCG305.316824E-447.62771266
AGGGGGG1351.4551915E-1146.9167570
TTAATCG306.3036726E-446.01080337
CCTATTA306.452052E-445.79409416
GCCCTAT306.455308E-445.78940614
TTATACA751.8189894E-1145.787062
AATGGTA2150.044.18903470
GGTATCA5000.043.9713331
AGCGTCA803.6379788E-1142.925373
GTGGTAT1650.041.639521
TATACAC856.91216E-1140.400353
GTATCAA12050.040.1968231
CTTAGTC458.366442E-539.5214165
AATCGTT350.001335641139.50081639
GACTTTA350.001358803439.36291533
TGTGTCG4700.039.08065470
TGGTATC1700.038.380332
ACTAGAT901.2551027E-1038.24986332
TACTAGA901.2551027E-1038.2380931
Produced by FastQC (version 0.11.5)