FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976817
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC67240.6883582083440398No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT57440.588032354064272No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC56980.5833231813123645No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT47650.48780887310519777No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44890.459553836593753No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTT44790.4585301033868166No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42810.43826018588947574No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCA42250.4325272799306318No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42080.43078693347884No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37550.3844118192046207No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG34920.35748763586219323No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG34250.35062862337571926No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33980.347864543716991No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33680.3447933440961818No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCAC33490.3428482510030026No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33140.3392651847787252No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG32920.3370129717234651No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32790.33568211855444774No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA30580.3130576146811532No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG29780.30486774902566194No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28400.2907402307699395No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC27740.2839835916041592No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATT26780.2741557528175697No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG25570.2617685810136392No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24710.2529644754339861No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG24590.2517359955856624No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24090.24661732955098037No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23950.2451841030612694No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT22610.2314660780883216No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22140.22665453201572044No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGG22140.22665453201572044No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA21590.2210239993775702No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC21190.21692906654982458No Hit
GTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGG21160.21662194658774364No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20830.21324362700485353No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20720.21211752047722346No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC20370.20853445425294603No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTAC20180.2065893611597669No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA20150.20628224119768596No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20100.20577037459421776No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC19960.2043371481045068No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19820.20290392161479584No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19780.20249442833202128No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC19740.2020849350492467No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC19420.19880898878705017No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAA19410.19870661546635654No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19000.19450930931791727No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAG18940.19389506939375545No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGT18330.18765029683144335No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18230.18662656362450694No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA17210.1761844849137556No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17190.1759797382723683No Hit
GACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACT16940.1734204052550273No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16690.1708610722376863No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16370.16758512597548977No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16310.16697088605132793No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15910.1628759532235823No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15900.16277357990288865No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC15360.1572454205854321No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG15340.1570406739440448No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15300.15663118066127024No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGGCCAGGCCC15290.1565288073405766No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG15280.15642643401988296No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15000.153559981040461No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14940.15294574111629916No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT14760.15110302134381362No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC14680.1502840347782645No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14600.1494650482127154No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14460.14803182172300441No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAACTGGGACCAAGGT14180.14516536874358246No Hit
ACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG14160.14496062210219518No Hit
GCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC14090.1442440088573397No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC13860.14188942248138597No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13770.14096806259514322No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC13770.14096806259514322No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13750.14076331595375594No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13720.140456195991675No Hit
AGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCC13210.13523515663629934No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC13200.1351327833156057No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13160.13472329003283112No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12690.12991174396023No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA12680.12980937063953638No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12660.1296046239981491No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA12610.12909275739468087No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC12290.12581681113248439No Hit
CCTCATAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCAT12270.1256120644910971No Hit
CCTTGACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGT12100.12387171803930522No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA12020.12305273147375607No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT11940.12223374490820697No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG11780.1205957717771087No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG11720.11998153185294688No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG11630.1190601719667041No Hit
GGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGG11620.11895779864601046No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11610.11885542532531683No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG11540.11813881208046133No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG11460.11731982551491221No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG11320.11588659902520124No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG11300.11568185238381395No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11180.11445337253549027No Hit
GTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACA11140.11404387925271571No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11040.1130201460457793No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10890.11148454623537468No Hit
AGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGC10800.11056318634913191No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC10670.10923233318011459No Hit
GCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGT10480.10728724008693544No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10480.10728724008693544No Hit
TAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTGTTGTCCT10480.10728724008693544No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10440.10687774680416086No Hit
GGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATCT10400.1064682535213863No Hit
CTCCAGAGCAGGTGATCCTGGCCGTTTGTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGG10340.10585401359722445No Hit
GCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCT10280.10523977367306261No Hit
AGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAG10280.10523977367306261No Hit
ACAGACAGCAGTGGTAATCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC10270.10513740035236897No Hit
CAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGT10180.1042160404661262No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG10100.10339705390057707No Hit
GATTACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCC10080.10319230725918979No Hit
TCTTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATC10000.10237332069364068No Hit
GAGTAACAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGT9980.1021685740522534No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG9850.10083772088323606No Hit
GCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATT9810.1004282276004615No Hit
GTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGG9810.1004282276004615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)