FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468250
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT13900.2968499733048585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3200.059.5765570
GTGGTAT2500.049.4246671
TATACAC2850.045.7489243
CTTATAC2800.045.354981
TTATACA2900.043.776992
TGGTATC2900.042.5938262
GGTATCA8400.042.494761
GTATCAA23150.042.106451
TCGTATG2800.039.6580140
CGTATGC3000.039.3530241
CTCGTAT2700.038.5314339
TATCAAC25950.037.2866942
ATACACA3650.035.721764
AAGGGGG3650.034.246469
TCAACGC28550.034.131424
ATCAACG28450.034.130793
AATCTCG3150.034.0396736
TCTCGTA3100.033.53429838
AACGCAG28650.033.533246
CAACGCA28800.033.4777265
Produced by FastQC (version 0.11.5)