FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523667
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT12870.24576687093133612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2200.077.9698270
GAGTTAG150.002267527669.6603342
TGACTCA200.007030648652.3467944
CGAGTTA200.00710661252.2047441
ATACACT200.007538228851.432114
CTTATAC2450.049.015721
ATACTCA250.00995307647.93845769
TCCATTG305.756005E-446.8629457
TTATACA2600.046.157022
ACTCTCA306.502759E-445.7174346
TATACAC2650.045.2861373
GTGTATA405.1833493E-542.86009213
TCGTATG2800.042.21597740
ATACACA2850.042.1081624
CTCGTAT2400.041.98464639
AGACTAA3000.041.1614125
GACTAAG3050.040.5021126
ACTAAGG3000.040.0486427
TACACAT3050.039.3469735
ATATCAA350.001384015839.212581
Produced by FastQC (version 0.11.5)