FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523667
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29350.5604706807952382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATC603.0167394E-740.0595131
GTATCAA26050.039.8068731
TATCAAC27800.037.0434042
AACGCAG29900.034.2120826
CAGAGTA29850.032.43871310
GGTATCA10750.032.2605481
ATCAACG31850.032.2252433
GCAGAGT31600.032.1635139
AGAGTAC30100.031.94113711
CAACGCA32800.031.1872335
CGCAGAG32800.031.0914848
TCAACGC33250.031.0748674
ACGCAGA32950.030.941087
AGAGTCG1950.028.17372311
GTGGTAT4700.027.7616081
TACAGGT500.00792324627.4615122
GAGTACG36450.025.62301412
CAGAGTC2150.025.55291210
AGTACGG36150.025.55070113
TGGTATC5600.025.1321852
Produced by FastQC (version 0.11.5)