FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185219
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT12210.10301893574098964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6750.040.7508351
GTATCAA43250.040.544731
TGGTATC7650.036.8228842
GGTATCA18100.036.0931971
TATCAAC49650.035.287132
TTATACA4100.035.1909372
CTTATAC4150.033.969261
TATACAC4250.033.9489023
TCGTATG3100.033.57636340
CAACGCA53250.032.8370065
ATCAACG53950.032.53833
CTCGTAT3100.032.42675439
AACGCAG54150.032.418126
TCAACGC55200.031.9881734
AGGGGGG6300.031.9528570
ATACACA4850.031.1656574
TACACAT5300.030.4640315
ACGCAGA58550.029.980657
AGAGTAC56900.029.82371511
CAGAGTA57150.029.7533610
Produced by FastQC (version 0.11.5)