FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741544
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT29150.39309872374397203No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT23600.318254884403353No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21190.28575512714012924No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20090.2709212130365831No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19210.2590540817537462No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT18330.24718695047090933No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA18160.2448944364730886No Hit
GTGTATTACTGTGCAACAGCGGGACTTGTTGGGCAGTGGCTGGTACCTCT17310.23343186648398478No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC16650.22453151802185708No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG16260.2192722212033271No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15900.2144174856785302No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATAAGGCGT15530.20942789638915563No Hit
GTTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGTCATGGTGACTCTG14890.20079725545618332No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13870.18704217146925875No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13340.17989492194664106No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAGGTTTTGATCCTGAAAATGGTGAAACAATCTACG13070.17625387030304338No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12920.174231063834378No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12880.17369164877606724No Hit
ATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGG12720.17153398854282415No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT12650.1705900121907803No Hit
AGCTGGTACAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTATCCGGAGACA12450.16789293689922646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12430.1676232293700711No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12340.16640954548887188No Hit
CTATTATAGCGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC12250.16519586160767263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12050.1624987863161188No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA11980.16155480996407495No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11740.15831831961421036No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11730.15818346584963267No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGT11490.15494697549976805No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG11210.15117107009159267No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA11100.14968767868123808No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA11090.14955282491666036No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT11030.14874370232919423No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA10880.14672089586052886No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA10820.14591177327306268No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT10770.14523750445017422No Hit
GGTCTAGAGGTACCAGCCACTGCCCAACAAGTCCCGCTGTTGCACAGTAA10490.14146159904199887No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA10460.14105703774826578No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10250.13822510869213425No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10220.13782054739840116No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9890.13337037316733735No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTTTCAGCAGAAA9750.13148242046324965No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9730.13121271293409426No Hit
GTATCCGGAGACACCCTCACTGAATTATCCATGCACTGGGTGCGACAGGC9690.13067329787578352No Hit
GAATTATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG9510.12824593011338503No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC9260.12487458599894274No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9190.12393060964689889No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA9150.12339119458858815No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9150.12339119458858815No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG9070.12231236447196658No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9070.12231236447196658No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9030.12177294941365584No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG9010.12150324188450044No Hit
CTGTAGATGTGTCCTCGGTCATGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTTCACCATTTTCAGGAT8970.12096382682618967No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8910.12015470423872353No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACCCCA8850.11934558165125739No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8840.11921072788667968No Hit
GTCATGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTTCACCATTTTCAGGATCAAAACCTCCCATCCA8840.11921072788667968No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8770.11826675153463584No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8760.11813189777005816No Hit
GTGCATGGATAATTCAGTGAGGGTGTCTCCGGATACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC8760.11813189777005816No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8740.11786219024090276No Hit
CCTCTAGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC8690.1171879214180143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8610.11610909130139277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8610.11610909130139277No Hit
CTATAATAGTTATTCTCCGACAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8590.11583938377223739No Hit
GGGTTCCCTGGCCCCAGTGGTCTAGAGGTACCAGCCACTGCCCAACAAGTCCCGCTGTTGCACAGTAATACACGG8590.11583938377223739No Hit
GTCTAGAGGTACCAGCCACTGCCCAACAAGTCCCGCTGTTGCACAGTAAT8410.11341201600983894No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGGTCCAGCTGGTA8390.11314230848068355No Hit
CATCTACAGACACAGCCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCAACAG8340.11246803965779509No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8340.11246803965779509No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCA8230.11098464824744046No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8180.110310379424552No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8170.11017552565997432No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG8170.11017552565997432No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8160.11004067189539664No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT7940.10707388907468741No Hit
GTCGCACCCAGTGCATGGATAATTCAGTGAGGGTGTCTCCGGATACCTTG7770.10478137507686665No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCA7680.10356769119566742No Hit
GTATTAGTAGCTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCT7580.1022191535498905No Hit
CACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAG7440.1003312008458028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)