FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851148
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23450.27551025203607366No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22660.26622866998453854No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21140.24837043616386337No Hit
AGCTGGTACAGTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGATACA18810.22099564353085477No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18490.21723601535808107No Hit
GTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA18260.2145337826089No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18230.21418131746770244No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATTTCCTG16740.19667554878822485No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGGCTGCTATCCCATGTT16680.19597061850582975No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16280.19127108328986264No Hit
CGTCCATACCGTACATCCAGTTAGCGGTCGCACAATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCT16190.19021368786627002No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGG15150.17799489630475546No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC15050.17682001250076368No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15030.1765850357399653No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC14590.17141554700240147No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT14200.1668335001668335No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14190.16671601178643433No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13900.16330884875485813No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13660.16048912762527787No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13230.1554371272681132No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13030.15308735966012962No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12990.15261740613853292No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12180.14310084732619943No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11990.14086856809861506No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11960.1405161029574175No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11880.13957619591422407No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11830.1389887540122282No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTA11790.13851880049063148No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11640.13675647478464378No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11630.13663898640424463No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11510.13522912583945448No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11450.1345241955570594No Hit
ATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATG11310.1328793582314709No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11190.13146949766668076No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11170.1312345209058824No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10730.12606503216831855No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10700.12571256702712102No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10650.12512512512512514No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTA10560.12406772970153253No Hit
CTGTAGATGTGTCCTCGGTCACGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTTCACCATCTTCAGGAT10320.12124800857195224No Hit
GCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGTCACGGTGACTCTG10070.11831079906197275No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10000.11748838039917853No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9700.11396372898720318No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG9690.11384624060680398No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9680.1137287522264048No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9570.11243638004201384No Hit
GGACAGAAGGTCACCATTTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATCTATCCTGGTACCAGCAG9510.11173144975961877No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGGTCCAGCTGGTA9350.10985163567323192No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG9280.10902921701043766No Hit
CCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGTCACGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTT9260.10879424024963931No Hit
GAATTATCCATCCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG9130.10726689130445No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9120.10714940292405081No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9090.10679693778285328No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAGGCTTTGATCCTGAAGATGGTGAAACAATCTACG8980.1055045655984623No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC8910.10468214693566806No Hit
GCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGTCACGGTGA8860.10409470503367217No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8850.10397721665327299No Hit
CATCTACAGACACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTATTGTGCGACCG8820.10362475151207545No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC8640.10150996066489024No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8590.10092251876289435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)