FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607571
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19320.31798752738363084No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCAA17750.2921469260382737No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15980.2630145283431895No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACT14800.24359292987980005No Hit
GTTATAATAGTGCTGACAGAAATAAACTGCAGAGTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT14720.24227621133990923No Hit
CTATTATAACTGGCCTCAGGCGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCAT14320.2356926186404552No Hit
GTCCCAGACCCACCGCCAGTGAACCTGGCCGGGATACCAGTGGCCCTGGT13720.2258172295912741No Hit
GTCTGAGGACTCTGCAGTTTATTTCTGTCAGCACTATTATAACTGGCCTC12560.20672481076285734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12320.2027746551431849No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12230.20129334678580776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11640.19158254755411303No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11550.19010123919673586No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11250.18516354467214532No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11150.1835176464972818No Hit
GTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC10930.17989667051258207No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGCC10840.1784153621552049No Hit
ATTATAACTGGCCTCAGGCGTTCGGCCAAGGGACCAAGGTGGAAATTAAA10510.1729838981781553No Hit
GTCTCCAGCCACCCTGTCTGTGTCTTCAGGGGAAAGAGTCATCCTCTCCT10470.17232553890820992No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCGGCCAGGTTCACTGGCGGTGGGTCTGGGACAGAGTTCA10370.17067964073334638No Hit
CTGGTATCCCGGCCAGGTTCACTGGCGGTGGGTCTGGGACAGAGTTCACT10330.17002128146340098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10140.16689407493116032No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10120.16656489529618762No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACTCTGCAGTTTATTTCTGTCAGCACTATTATAACTGG10090.16607112584372855No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10080.1659065360262422No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10030.16508358693881045No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10020.16491899712132407No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9600.15800622478689733No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9560.15734786551695193No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9430.15520819788962936No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9220.15175181172241597No Hit
CTCCTGTGCAGCGTCTGGATTCGATTTCAAAATATATGACATGCACTGGATCCGCCAGGCTCCAGGCAAGGGA8980.14780165610274354No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCACCTTTTTGTTGATACCAGG8820.14516821902296193No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8690.14302855139563936No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG8570.14105347358580314No Hit
GTCTGTGTCTTCAGGGGAAAGAGTCATCCTCTCCTGCAGGGCCAGTCAGA8540.14055970413334407No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8480.13957216522842597No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8380.13792626705356248No Hit
GTGTATCTGCAAATGAACAGCCTGAGAAGTGAGGACACGGGTGTCTATTA8290.1364449586961853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8240.13562200960875354No Hit
GTAATAGACACCCGTGTCCTCACTTCTCAGGCTGTTCATTTGCAGATACA8090.13315316234645827No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7960.1310134947191357No Hit
GGACTGGAGTGGATGACATTTGTAGGGCCTGACGAAGTCAGAAAATATTA7860.12936759654427218No Hit
GTCTATTACTGTGGGAGTGTAGTTGACGGTATGGACGTCTGGGGCCAAGG7830.12887382709181314No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7790.12821546782186774No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7770.12788628818689501No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7770.12788628818689501No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7540.12410072238470894No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCACCTTTTTGTTGATACCAGGC7460.12278400384481813No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7300.12015056676503652No Hit
AGCTTAGCCTGGTATCAACAAAAAGGTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCA7120.1171879500502822No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCAGTGAACCTGGCCGGGATACCA7080.11652959078033678No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7070.11636500096285043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6960.11455451297050057No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT6760.11126271662077354No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6670.10978140826339639No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6620.10895845917596463No Hit
CTGCTACTCTGGCTCCCAGATGCAACTGGAGAAGTAGTGATGACGCAGTC6550.10780633045356015No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6550.10780633045356015No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6510.10714797118361474No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6430.10583125264372394No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6410.10550207300875124No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6300.10369158501640138No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6270.10319781556394232No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6220.10237486647651058No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG6110.1005643784841607No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6080.10007060903170165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1400.046.7878951
GTGTAAG306.3314015E-445.9670561
TAGGGTG451.0096359E-438.2775045
CTGATTA400.002494869334.77891550
TAGATTT400.002513224734.7269847
GAATAGA601.3481049E-534.4752921
CAACGCA11850.031.6879625
AACGCAG11850.031.6879626
ATCAACG12050.031.1620223
TCAACGC12300.030.8087254
AGAGTAC12500.029.48899311
ACGCAGA12800.029.3361237
CGCAGAG13100.028.9272778
GAGTTTA2400.028.729411
TGTTAGA2702.5465852E-1128.34740370
TATAAGA1900.027.1971782
CAGAGTA13600.027.10385310
TACTCCG658.726319E-426.4998135
GAGCCAT8000.026.30993370
TAAGAGA2100.026.2474354
Produced by FastQC (version 0.11.5)