FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607571
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19930.3280275062502983No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCAA15840.2607102708983806No Hit
GTTATAATAGTGCTGACAGAAATAAACTGCAGAGTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT14590.24013654371258666No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACT13910.2289444361235148No Hit
CTATTATAACTGGCCTCAGGCGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCAT13600.2238421517814379No Hit
GTCCCAGACCCACCGCCAGTGAACCTGGCCGGGATACCAGTGGCCCTGGT12510.2059018616754256No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12120.19948285879345787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12110.19931826897597155No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11900.19586188280875816No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11740.19322844572897654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11670.19207631700657207No Hit
CTGGTATCCCGGCCAGGTTCACTGGCGGTGGGTCTGGGACAGAGTTCACT11590.19075959846668128No Hit
GTCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC11150.1835176464972818No Hit
GTCTGAGGACTCTGCAGTTTATTTCTGTCAGCACTATTATAACTGGCCTC11150.1835176464972818No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10820.1780861825202322No Hit
ATTATAACTGGCCTCAGGCGTTCGGCCAAGGGACCAAGGTGGAAATTAAA10390.1710088203683191No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10230.16837538328853746No Hit
GTCTCCAGCCACCCTGTCTGTGTCTTCAGGGGAAAGAGTCATCCTCTCCT9950.16376686839891963No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9910.16310850912897423No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9880.16261473967651516No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9780.16096884150165167No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCGGCCAGGTTCACTGGCGGTGGGTCTGGGACAGAGTTCA9570.15751245533443828No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9500.15636032661203383No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9490.15619573679454749No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGCC9410.15487901825465666No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9230.15191640153990232No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9110.1499413237300661No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8960.14747247646777084No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGACTCTGCAGTTTATTTCTGTCAGCACTATTATAACTGG8910.1466495273803391No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8750.14401609030055745No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8690.14302855139563936No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8680.142863961578153No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG8500.1399013448633987No Hit
GTGTATCTGCAAATGAACAGCCTGAGAAGTGAGGACACGGGTGTCTATTA8470.13940757541093962No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCACCTTTTTGTTGATACCAGG8410.13842003650602153No Hit
GTCTGTGTCTTCAGGGGAAAGAGTCATCCTCTCCTGCAGGGCCAGTCAGA8270.13611577906121258No Hit
CTCCTGTGCAGCGTCTGGATTCGATTTCAAAATATATGACATGCACTGGATCCGCCAGGCTCCAGGCAAGGGA8190.1347990605213218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8180.13463447070383544No Hit
GTAATAGACACCCGTGTCCTCACTTCTCAGGCTGTTCATTTGCAGATACA8170.1344698808863491No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8020.13200103362405383No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7970.13117808453662205No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7920.1303551354491903No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7830.12887382709181314No Hit
GTCTATTACTGTGGGAGTGTAGTTGACGGTATGGACGTCTGGGGCCAAGG7640.12574662055957245No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT7540.12410072238470894No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7390.12163187512241368No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGATCCAGTGCATGTCATATATTT7300.12015056676503652No Hit
GGACTGGAGTGGATGACATTTGTAGGGCCTGACGAAGTCAGAAAATATTA7290.11998597694755017No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7180.11817548895520029No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7130.11735253986776854No Hit
CTGCTACTCTGGCTCCCAGATGCAACTGGAGAAGTAGTGATGACGCAGTC7110.11702336023279584No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCACCTTTTTGTTGATACCAGGC6920.11389615370055516No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6690.11011058789836907No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCAGTGAACCTGGCCGGGATACCA6640.10928763881093732No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6350.10451453410383314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6290.10352699519891502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6270.10319781556394232No Hit
AGCTTAGCCTGGTATCAACAAAAAGGTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCA6170.10155191738907882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1650.043.8128281
TCAACGC11400.033.4991534
CAACGCA11450.033.352875
ATCAACG11450.033.0523953
AACGCAG11650.032.7802856
TATAAGA1305.456968E-1231.7580342
AGAGTAC12500.030.84931211
GTATTGG450.004670867730.5994361
GTATAAG1359.094947E-1230.5994341
ACGCAGA12600.030.5893617
TGTTAGA2201.8571882E-929.74213670
CGCAGAG12850.029.7362218
CAGAGTA13150.029.3244410
CCGATTT605.2963704E-428.86284841
TCCGATT605.309225E-428.85089740
CTCCGAT500.00760714327.69227639
ATACAGT500.00781534227.5394941
ATAAGAG1457.239578E-1026.0999933
AGTACGG13350.025.27448313
GAGTACG13400.024.92323512
Produced by FastQC (version 0.11.5)