FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429064
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6380.14869576566666046No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC5660.13191505229988998No Hit
GTGCATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT5340.12445695747021422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4700.10954076781086272No Hit
AGCATGCACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA4450.10371413122517853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC200.00733171351.79310232
GTGTATA405.0866714E-542.9948541
GGTATCA4950.042.3868451
GTATCAA14050.041.3729821
ATGTAAG350.001367033939.309581
TGGTATC1750.039.291262
GTGGTAT1900.036.2061961
AGTAATC400.002385564835.09558559
GATAAGA400.002639295334.3798522
TATCAAC17350.033.686312
CAACGCA17250.033.682295
AACGCAG17250.033.682296
ATCAACG17600.033.0124663
TCAACGC18150.032.390934
CTTGTAC652.3740995E-531.7352493
AGAGTAC17700.031.68269511
CAGAGTA18050.031.64015810
GCAGAGT18400.031.599249
ACGCAGA18600.031.4297717
CGCAGAG18650.031.1720228
Produced by FastQC (version 0.11.5)