FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633349
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6780.10704998350040815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13350.044.59331
TATCAAC15250.038.351382
GGTATCA4950.037.539921
CAACGCA16800.034.6082275
ATCAACG16850.034.505533
TCAACGC16950.034.5049254
AACGCAG17450.033.319096
ACGCAGA17700.032.661857
AGAGTAC18150.031.67746511
ACGTATG450.004371479631.0167354
CAGAGTA18650.031.01280610
CGCAGAG18650.031.0079068
TAGCGTC450.0046849330.580845
GCAGAGT19450.029.7372139
TATGTAC500.00783881327.5227533
AGTACGG17800.027.07812713
GTGGTAT2300.026.930811
GAGTACG18150.026.5559612
TGGTATC2250.025.9937112
ACGGGTA951.06892985E-525.3619216
Produced by FastQC (version 0.11.5)