FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749599
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10100.13473870696198903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.040.9103161
GTATCAA16500.040.70371
CGTACCG350.001328479739.5427939
TATCAAC18800.035.5260542
TCAACGC19450.033.9884154
ATCAACG19650.033.6424753
CAACGCA19750.033.4721375
CGTATGC2150.032.2602841
GTGGTAT1700.032.0865251
AACGCAG21100.031.492056
TCGTATG2200.031.48861540
CTTATAC2300.031.1274151
CTCGTAT2050.030.38043239
TGTACCG450.004909823630.2897875
CGCAGAG22500.029.3810968
ACGCAGA22900.029.0166917
AGAGTAC23250.028.14019211
AGTACGG16850.027.5034913
CAGAGTA23800.027.34671610
CGGAATA500.00821414527.2608114
Produced by FastQC (version 0.11.5)