FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749599
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23160.3089651933900659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16600.042.374421
TATCAAC19150.036.192282
TCAACGC20100.034.648424
ACCGTAT701.0930635E-633.990498
CAACGCA20600.033.8074385
ATCAACG20900.033.3221633
GGTATCA6150.033.1526031
AACGCAG21400.032.7023546
CGCAGAG22550.031.2019128
ACGCAGA22700.030.9853947
AGAGTAC22600.030.38899411
GCAGAGT23800.029.4281969
CAGAGTA23350.029.41290310
CGAATTA500.00829651927.20510115
ACGGGTA756.798966E-527.20328516
TACGGGT2950.026.51344515
GTGTATA3100.026.3081931
AGTACGG17700.026.12570413
GAGTACG19150.024.50262612
GTGGTAT2900.023.435461
Produced by FastQC (version 0.11.5)