FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972323
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22650.23294728192174824No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21190.2179316955373883No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20590.21176090661230887No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT19700.20260756970677438No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16950.1743247871334937No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.1633202135504354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15840.16290882762209677No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15470.15910350778496446No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13960.14357368899018125No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13820.14213383824099604No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13820.14213383824099604No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA13340.1371972071009325No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12990.1335975802279695No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12870.13236342244295363No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGC12490.12845525612373668No Hit
CTCCTGCAGGGCCAGTCAGAGTCTGACCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT11790.12125600237781066No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11480.11806776143318629No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT11450.11775922198693234No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACCGGAGGCCAGTTACTATATTG11370.11693645013025507No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11300.11621652475566246No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11180.11498236697064658No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11120.11436528807813864No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10950.11261689788269948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10900.1121026654722762No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10860.11169127954393757No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10850.1115884330618529No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10820.11127989361559892No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGG10510.10809165267097456No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTCT10420.10716603433221265No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10380.10675464840387403No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGCAATATAGTAACTGG10130.1041834863517576No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTGACCAC10070.10356640745924967No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9970.10253794263840309No Hit
GTCAGAGTCTGACCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG9950.10233224967423377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9860.10140663133547186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9830.10109809188921788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27000.037.2594381
TCGCTAT400.002645312834.3682324
TATCAAC30600.032.751982
TTAACGG751.6723607E-632.1913935
GTAGAAC553.3567913E-431.202073
AACGCAG32200.030.6981876
ATCAACG32750.030.6018453
TGTATCA450.00473345330.51810818
TCAACGC32900.030.4623284
CAACGCA32600.030.4268045
AGAGTAC34500.028.37215211
CGCAGAG34650.028.3397038
GCAGAGT35800.027.6282679
TAACGGC756.160724E-527.6011836
TTTAACG756.184625E-527.58549534
ACGCAGA36150.027.3481067
TCTAAAG658.9211366E-426.401752
CAGAGTA37650.026.18083210
TAGTTTA700.00122360825.0097856
GTATTAG1251.3242243E-724.7209381
Produced by FastQC (version 0.11.5)