FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048756
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGCGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA30580.29158355232294264No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23230.22150052061680695No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22720.21663761637597304No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21740.20729321214848828No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18590.17725762713157303No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18340.17487385054292895No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17950.1711551590646442No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGGGGTGATG17280.1647666378070781No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGGGGTGATGCTGCTTGTATATGAG16150.1539919676264069No Hit
GTATATTACTGTGCGAAAGACTCTGCAACCTGGTTTTACTATGATAGTAGTGGTTATTACTTCCCCTCTTTTGA15620.14893836125848148No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15580.14855695700429844No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15430.14712669105111198No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15150.14445686127183063No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14770.14083352085709164No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14530.13854509533199333No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14330.13663807406107809No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14250.13587526555271198No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14080.134254297472434No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13490.12862858472323402No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13250.12634015919813568No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12940.12338427622821706No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATTACCATCTCCTG12910.12309822303757977No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12530.11947488262284078No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12520.11937953155929501No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12140.11575619114455601No Hit
CCACAGGGGTGATGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCG11310.10784205287025772No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11150.10631643585352551No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11080.10564897840870517No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG11050.10536292521806788No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT10990.1047908188367933No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10870.10364660607424414No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10750.102502393311695No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG10610.10116747842205433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG652.0372681E-1047.993140
GTATCAA11900.039.7143141
CTTATAC1009.094947E-1237.9460141
GGTATCA5500.037.0052031
CGTATGC904.9349183E-934.67830341
CTCGTAT904.989488E-934.63844339
GTATACC400.002601824234.4848633
TATCAAC13800.033.2354132
TCAACGC15000.030.1167814
TATACAC1501.8189894E-1229.886883
TCTGGAG15800.029.79676270
ATCAACG15250.029.6230623
TACGTTG605.0702767E-429.0812356
AATATAC10050.028.823173
AACGCAG15800.028.8101396
CTAGGCA1202.6775524E-928.7565828
GTAATAT10400.028.525851
TATGCCG1103.4626282E-828.3908543
CAACGCA15800.028.3736215
TAATATA10350.028.3209022
Produced by FastQC (version 0.11.5)