FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810225
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGAGCCGTGTCTGCGGCGGTCATAGAGTTCAGGCTCAGGGAGA11060.13650529173994877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10870.13416026412416304No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10330.12749544879508778No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10310.12724860378289982No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10150.12527384368539604No Hit
CTTTATTACTGTGCGACACATAGCCAACTGGGAATTAACTACTGGGGCCA8870.10947576290536579No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8640.10663704526520411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)