FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521578
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25710.4929272323602606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT150.002442742568.365076
CTATACG300.002979067665.01787670
TAGCGTC306.592911E-445.5898257
GTGGTAT3450.039.6471251
TATTATG350.001367752439.30692332
GGTATCA8500.035.0002481
TGGTATC3850.034.626462
GTATCAA18600.034.563341
TATCAAC20600.031.195712
TCCTATA450.00483411630.3844742
GTCTAGC802.9550174E-629.921191
AACGCAG23100.026.9316946
ATCAACG23900.026.6023083
CAACGCA23700.026.538345
TCAACGC23850.026.5147534
AGAGTAC22950.026.37564311
ACGCAGA23600.026.3686897
CAGAGTA23200.025.94401210
GCAGAGT24700.025.3376669
CGCAGAG24600.025.2992178
Produced by FastQC (version 0.11.5)