FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851263
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37870.4448683896751062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25650.3013169842927509No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23950.2813466578483971No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22970.26983435201576955No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22610.26560534170990635No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22270.2616112764210356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21020.24692721285901068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21010.2468097403505145No Hit
GTGCAGCCACAGTTCGTTTTATCTCCAGTCGTGTCCCTTGGCCGAAGGTG19920.23400523692442876No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCAAGTCAGAGCCTCCTG19830.23294798434796296No Hit
GCTCTACACATTCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATAAAACGAACTGTGGCTGCACCAT19030.223550183668267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18940.22249293109180124No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18570.21814644827744187No Hit
GTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC17540.20604677990233333No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCGGGCACAGATTTTACACTGAA17160.20158282457947782No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCCGATCCACTGCCA16250.19089282630632365No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15920.1870162335259491No Hit
GTAATGGATACAACTATTTAGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT15700.1844318383390327No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15450.18149502562662773No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15060.17691359779527596No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14380.1689254672175344No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC14280.16775074213257243No Hit
GTTCAGTGGCAGTGGATCGGGCACAGATTTTACACTGAAAATCAGCAGAG14220.16704590708159522No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14000.16446151189467884No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACACATT13080.15365404111302852No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12860.15106964592611216No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT12830.15071722840062354No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12620.14825030572220335No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12600.14801536070521096No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12530.14719305314573758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12480.14660569060325657No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12470.14648821809476037No Hit
CTACACATTCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATAAAACGAACTGTGGCTGCACCAT12040.14143690022942382No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11980.1407320651784466No Hit
GCAGTGGATCGGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT11810.13873503253401123No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11360.13344876965168226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11180.13133426449875069No Hit
GGACACGACTGGAGATAAAACGAACTGTGGCTGCACCATCTGTCTTCATC11130.13074690195626967No Hit
TTATTACTGCATGCAAGCTCTACACATTCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATAAAACGAA11120.13062942944777348No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTTATCTCCAGTCGTGTCC10980.1289848143288267No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAC10900.12804503426085712No Hit
CCTTCGGCCAAGGGACACGACTGGAGATAAAACGAACTGTGGCTGCACCA10790.12675283666739892No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10580.12428591398897873No Hit
GTTTATTACTGCATGCAAGCTCTACACATTCCTCCGATCACCTTCGGCCA10510.12346360642950534No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA10510.12346360642950534No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10490.12322866141251294No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC10470.12299371639552055No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10370.12181899131055854No Hit
GCTAATGCTCTGGGTCTCTGATTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC10160.11935206863213836No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10040.11794239853018397No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACACAT9870.1159453658857486No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9740.11441822327529799No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCCGATCCACTGCCA9570.11242119063086262No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9510.11171635557988542No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGAATGTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAG9390.11030668547793103No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT9320.10948437791845764No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9120.10713492774853364No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9110.10701745524003745No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8910.10466800507011347No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGATTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT8910.10466800507011347No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8810.10349327998515148No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8760.10290591744267048No Hit
ATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGA8720.1024360274086857No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8690.10208360988319708No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8660.1017311923577085No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8550.10043899476425029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGT300.003396995462.9060370
GTGGTAT1850.046.3912471
GTATCAA13750.040.9457551
GGTATCA5800.037.8808561
TATCAAC15150.036.9136962
TCAACGC16550.033.9983984
TGGTATC2550.033.6366232
ATCAACG16750.033.5924453
AACGCAG16900.033.294296
CAACGCA16900.033.0912745
TCGTATG1101.020453E-931.46945440
TATAGTC450.004749014530.4972065
AGAGTAC18600.029.88409811
CGCAGAG19100.029.2797138
CAGAGTA19100.029.10179110
TACACCG953.361274E-728.8920945
TGTTAGA6700.028.16687870
ACGCAGA19900.028.1009887
CTTATAC1503.6379788E-1127.4636171
CCTAAGA1155.8358637E-826.90295229
Produced by FastQC (version 0.11.5)