FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851263
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37400.43934718177578497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22390.26302094652298996No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22160.2603190788275774No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21850.2566774310641952No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21630.25409303587727883No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20090.23600226956886414No Hit
GTGCAGCCACAGTTCGTTTTATCTCCAGTCGTGTCCCTTGGCCGAAGGTG18890.22190556854932023No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17660.20745645000428775No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCAAGTCAGAGCCTCCTG17500.20557688986834857No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17460.20510699983436378No Hit
GCTCTACACATTCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATAAAACGAACTGTGGCTGCACCAT17210.20217018712195878No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17150.2014653520709816No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCCGATCCACTGCCA16680.19594414417166023No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCGGGCACAGATTTTACACTGAA16050.18854337613639968No Hit
GTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC15990.18783854108542247No Hit
GTAATGGATACAACTATTTAGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT15860.18631139847497188No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15780.18537161840700228No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15390.18079019057565054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15310.17985041050768094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14860.17456414762535197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14160.16634107203061804No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC14040.16493140192866362No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACACATT13730.16128975416528146No Hit
GTTCAGTGGCAGTGGATCGGGCACAGATTTTACACTGAAAATCAGCAGAG13520.1588228314868613No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12690.14907261328167676No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT12580.14778041568821856No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12580.14778041568821856No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12540.14731052565423378No Hit
GCAGTGGATCGGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT12440.14613580056927178No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12410.1457833830437832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12260.14402129541634018No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12110.1422592077888972No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11920.14002723012746943No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11780.1383826150085226No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11590.13615063734709484No Hit
CTACACATTCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATAAAACGAACTGTGGCTGCACCAT11590.13615063734709484No Hit
TTATTACTGCATGCAAGCTCTACACATTCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATAAAACGAA11550.13568074731311006No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11350.13333129714318606No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11190.13145173700724688No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10470.12299371639552055No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10350.12158404629356616No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10330.12134910127657376No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTTATCTCCAGTCGTGTCC10310.12111415625958134No Hit
GGACACGACTGGAGATAAAACGAACTGTGGCTGCACCATCTGTCTTCATC10260.12052679371710037No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAC10210.11993943117461936No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCAA9930.11665020093672579No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9860.1158278933772524No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9830.11547547585176378No Hit
GTTTATTACTGCATGCAAGCTCTACACATTCCTCCGATCACCTTCGGCCA9760.11465316829229039No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9760.11465316829229039No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC9700.11394833324131322No Hit
ATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGA9510.11171635557988542No Hit
CCTTCGGCCAAGGGACACGACTGGAGATAAAACGAACTGTGGCTGCACCA9450.11101152052890823No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9380.11018921296943483No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGAATGTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAG9350.10983679544394624No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9300.10924943290146524No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9220.10830965283349565No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9210.10819218032499946No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT9020.10596020266357166No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGATTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC8840.10384569751064007No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCCGATCCACTGCCA8700.10220108239169327No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8640.10149624734071608No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8610.10114382981522749No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8580.10079141228973891No Hit
GCTAATGCTCTGGGTCTCTGATTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC8570.10067393978124269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)