FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944768
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36570.3870791559409294No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC29530.31256350765478935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27410.29012413629589484No Hit
GTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTTATTTATAAGGTTT26420.2796453732556564No Hit
CCCTTGGACAGCCGGCCTCCCTCTCCTGCAGGTCTAGTCAAAGCCTCGTA26050.2757290678769815No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24750.26196907600596125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24470.25900538544912616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23810.2520195434223005No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22410.23720109063812492No Hit
GAACACACTGGCCCCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21490.22746325023709524No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20840.22058325430158515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19910.2107395678092399No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC19690.20841095380029806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18810.19909649776453053No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.196238653299011No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAG18280.19348665492480693No Hit
CTCGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCC18120.1917931174637583No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17860.18904111908955426No Hit
GTCTGGGACCCCAGAGTCCCGGTAAGAAACCTTATAAATAAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA17790.1883001964503455No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT17190.18194943097141308No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16740.1771863568622138No Hit
CGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAG16560.1752811272185341No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16540.175069435035903No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT16540.175069435035903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16310.17263497493564559No Hit
CTCCATCACTGTATACGAGGCTTTGACTAGACCTGCAGGAGAGGGAGGCC16040.169777130470126No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15960.1689303617396017No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15780.16702513209592196No Hit
GGCCAGGCCAATCTCCAAGGCGCCTTATTTATAAGGTTTCTTACCGGGAC15620.16533159463487332No Hit
GTGTGTTCCTTGCAGGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC15530.16437897981303348No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14710.1556996003251592No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGAGGGAGGCCGGCTGTCCAAGGGTGACGGACAGGGAGAGTGGAGA14450.15294760195095516No Hit
GTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCCTCTCCTGCAGGTCTAGTCAAAGCCTCGTATA14270.15104237230727544No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14250.15083068012464437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14120.14945468093754236No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGT13820.14627929819807614No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13790.14596175992412952No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCACTGCCG13490.1427863771846633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13480.1426805310933478No Hit
GTCCAAGGGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAACATCC13370.14151622408887685No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT13310.1408811475409836No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACT13250.14024607099309036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13210.1398226866278282No Hit
GGAACACACTGGCCCCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12750.13495376642731338No Hit
GATTTCACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCCTGCAAGGAACACACTGG12690.13431868987942014No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12660.13400115160547352No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12630.1336836133315269No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC12610.1334719211488958No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12600.13336607505758027No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12570.13304853678363365No Hit
CTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAGG12360.1308257688660073No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12310.13029653840942962No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12060.12765038612654112No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGA11910.126062694756808No Hit
AGTGTGTTCCTTGCAGGCAGTAATAAACTCCAACATCCTCAGCCTCCACC11860.12553346430023032No Hit
GCCTGAGAGTCGACGACACGGCCGTATATTATTGTGCGAAAGGCCGCAGTGGCTTTGATTACTGGGGCCAGGGAA11810.1250042338436526No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11780.124686695569706No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11670.12352238856523506No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11650.12331069638260399No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11390.12055869800839994No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC11330.1199236214605067No Hit
GTATATTTGCGATTGACCAGCCTGAGAGTCGACGACACGGCCGTATATTA11240.11897100663866683No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG11130.1178066996341959No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11130.1178066996341959No Hit
GTGAAGGGCCGCTTCACCGTCTCCAGAGACAATTCGATTGCCACGGTATA11080.11727746917761821No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGACTCCATCACTGTAT10720.11346700989025876No Hit
GTTTATTACTGCCTGCAAGGAACACACTGGCCCCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10670.11293777943368108No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10650.11272608725105No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10530.11145593415526352No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGC10460.11071501151605474No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGAGGGAGGCCGGCTGTCC10440.11050331933342365No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC10340.10944485842026826No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10300.10902147405500609No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10280.10880978187237501No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10180.10775132095921962No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10150.107433782685273No Hit
ATAATATACGGCCGTGTCGTCGACTCTCAGGCTGGTCAATCGCAAATATA10120.10711624441132638No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9980.10563439913290883No Hit
CCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCCTCTCCTGCAG9940.10521101476764665No Hit
CTCTTGTGTAGCCTCTGGATTCACCTTTAGCAGATATGAAATGAGTTGGATTCGCCAGGCTCCAGGGAAGGGG9880.10457593821975343No Hit
ATTTATAAGGTTTCTTACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACA9750.1031999390326514No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9750.1031999390326514No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCCTGCAAGGAACACACTGGCCCCCGACTTTCGGCGGAGGGA9600.1016122476629183No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9480.10034209456713182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)