FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944768
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37150.3932182292372307No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27780.29404044167456983No Hit
GTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTTATTTATAAGGTTT23960.2536072347920336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23160.2451395474867904No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22380.2368835523641783No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22240.23540170708576075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21560.228204172876304No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20570.21772540983606556No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20570.21772540983606556No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC20300.214867565370546No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT20120.21296233572686626No Hit
CCCTTGGACAGCCGGCCTCCCTCTCCTGCAGGTCTAGTCAAAGCCTCGTA20080.21253895136160414No Hit
GAACACACTGGCCCCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA19100.2021660344126812No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17850.18893527299823873No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17790.1883001964503455No Hit
CTCGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCC17500.18523065980219483No Hit
GTCTGGGACCCCAGAGTCCCGGTAAGAAACCTTATAAATAAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA17260.18269035361062186No Hit
GTGTGTTCCTTGCAGGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC16810.1779272795014226No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16730.17708051077089823No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAG16460.17422266630537867No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16310.17263497493564559No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15820.1674485164611841No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15050.15929836742988754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15050.15929836742988754No Hit
CGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAG14530.15379437068147947No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCACTGCCG14460.1530534480422707No Hit
CTCCATCACTGTATACGAGGCTTTGACTAGACCTGCAGGAGAGGGAGGCC14440.1528417558596396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14390.15231252540306192No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT14080.14903129657228018No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14020.14839622002438693No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13920.14733775911123154No Hit
GTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCCTCTCCTGCAGGTCTAGTCAAAGCCTCGTATA13630.14426822246308088No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13440.14225714672808562No Hit
GGCCAGGCCAATCTCCAAGGCGCCTTATTTATAAGGTTTCTTACCGGGAC13340.14119868581493022No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13270.14045776317572145No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13190.13961099444519712No Hit
GTCCAAGGGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAACATCC13010.13770576480151742No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13000.13759991871020186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12960.1371765343449397No Hit
GATTTCACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCCTGCAAGGAACACACTGG12890.13643561170573093No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGAGGGAGGCCGGCTGTCCAAGGGTGACGGACAGGGAGAGTGGAGA12700.13442453597073567No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA12450.13177838368784717No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12410.13135499932258501No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGT12310.13029653840942962No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12150.12860300094838098No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACT12000.12701530957864787No Hit
AGTGTGTTCCTTGCAGGCAGTAATAAACTCCAACATCCTCAGCCTCCACC11920.12616854084812357No Hit
GCCTGAGAGTCGACGACACGGCCGTATATTATTGTGCGAAAGGCCGCAGTGGCTTTGATTACTGGGGCCAGGGAA11870.12563931039154586No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11640.12320485029128844No Hit
CTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAGG11420.12087623628234656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11340.12002946755182226No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11320.11981777536919118No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11260.11918269882129794No Hit
GGAACACACTGGCCCCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11190.11844177618208915No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC11120.11770085354288037No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11100.1174891613602493No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT11000.11643070044709389No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG10950.11590146999051619No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10950.11590146999051619No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10890.11526639344262295No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT10830.1146313168947297No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGA10790.11420793252946757No Hit
GTTTATTACTGCCTGCAAGGAACACACTGGCCCCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10790.11420793252946757No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10720.11346700989025876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10710.11336116379894325No Hit
GTATATTTGCGATTGACCAGCCTGAGAGTCGACGACACGGCCGTATATTA10600.1121968567944723No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10580.11198516461184121No Hit
GTGAAGGGCCGCTTCACCGTCTCCAGAGACAATTCGATTGCCACGGTATA10550.1116676263378946No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA10360.10965655060289933No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCCTGCAAGGAACACACTGGCCCCCGACTTTCGGCGGAGGGA10240.10838639750711286No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC10210.10806885923316624No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGAGGGAGGCCGGCTGTCC10080.10669286004606421No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA9980.10563439913290883No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTT9890.10468178431106896No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9880.10457593821975343No Hit
GCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCACTGCC9820.10394086167186019No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9760.10330578512396695No Hit
CTCTTGTGTAGCCTCTGGATTCACCTTTAGCAGATATGAAATGAGTTGGATTCGCCAGGCTCCAGGGAAGGGG9750.1031999390326514No Hit
GTCCACGTACCACTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGACCTCAGGGGT9720.10288240075870478No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAGTCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC9610.10171809375423384No Hit
CTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCCTCTCCTGCAGGTCTA9500.1005537867497629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)