FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055251
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19830.1879173770031964No Hit
GTAATACACAGCCGTGTCTTCATCTTTCAGGTTGTTCATTTGCAGATATA17160.16261533985753154No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAACA17160.16261533985753154No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGTCTACAGTCTGAAGACT16040.15200175124212156No Hit
CTTCTATCCCAGCCAGATTCAGTGGCGGTGGGTCTGGGACAGAGTTCACT14810.14034575660198378No Hit
CACTTATAGTGTCTGGCGACGAAACTAAGTTTTATGCAGACTCGGCGAAG13850.13124839493163237No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGGAGCATGATGGTTGGGCAC13420.12717353501678746No Hit
GTCCCAGACCCACCGCCACTGAATCTGGCTGGGATAGAAGTGGCCCTGGT13160.1247096662310673No Hit
GTGTATTACTGTGCGAGGAGAGCACTTTACGGTATGGACGTCTGGGGCCC13090.12404631694260418No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT12750.12082433468435473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12540.11883428681896534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12480.11826570171456839No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12140.11504371945631892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11140.10556730104970286No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10760.10196626205518876No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10580.10026050674199788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19550.039.406871
GTGGTAT3750.036.685921
TATCAAC22850.033.5477182
ATCAACG24350.031.4811213
TCAACGC24850.030.8476964
CAACGCA25250.030.359025
AACGCAG25300.030.2990236
ACGCAGA27100.028.2972687
AGAGTAC27900.027.13154411
CGCAGAG28750.026.6795738
GACAATT4850.026.37717470
CAGAGTA29250.025.99695210
GTATAAG2600.025.1333831
TTAACGG700.001325068324.67055535
TGTATTA6500.023.7981622
GAGACAA7000.023.75829770
GTATTAG1452.0820153E-823.7193431
GTGTATA1803.5652192E-1022.92871
TACGGTA6650.022.79087828
TTACGGT6700.022.6218727
Produced by FastQC (version 0.11.5)