FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796163
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAACA28880.3627397907212468No Hit
GTATATTACTGTGTGAAGGCCGCGGGCCACGGTGACAACCACGAGTCTCT18670.23449971927858992No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18450.23173646602517323No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18240.2290988151923664No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17100.21478013924284348No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16950.21289610293369574No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15350.19279971563611972No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14570.18300272682855143No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14040.1763457985362294No Hit
CTCCTGTGCAGTGTCTGGATTCACCTTCAGTACCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG13490.16943766540268762No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGTGACAGAATTCA13370.16793043635536944No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13340.16755362909353988No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13250.16642320730805124No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12530.15737983302414202No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12510.15712862818292234No Hit
ATACTACACAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATT12020.1509741095730397No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11960.1502204950493806No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11950.15009489262877074No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11620.1459500127486457No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11590.14557320548681613No Hit
GCACTGAACACAGAGGACTCAGCATGGAGTTTGGGCTGAACTGGGTTCTC11080.1391674820357138No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10730.13477139731436905No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10720.1346457948937592No Hit
CTACACAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATTTCAAGAACACACTGTTTCTGCAAATGAA10500.1318825416403425No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9700.1218343479915545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9630.12095513104728554No Hit
CAGTAATATACAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAA9550.11995031168240673No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9470.11894549231752795No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA8760.11002772045422858No Hit
GTGTAGTATTCATTACTTGCATCATACCAAATAACAGCCAGCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACC8600.10801808172447099No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8530.10713886478020206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8420.1057572381534937No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8260.1037475994237361No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA8230.10337079216190653No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8220.10324518974129668No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC8210.10311958732068685No Hit
GCACTAGAAGTCGGCGGTGTCTCCATTCGGTGATCAGCACTGAACACAGA8170.10261717763824743No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8140.1022403703764179No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8130.10211476795580804No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGGTAC7980.1002307316466603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA150.002251575269.78792661
GTATCAA10900.042.8154261
TATCAAC12400.037.6219562
TGACTTG3950.037.4653870
GTGGTAT1950.037.2286831
TATAAGA1450.035.7480662
ATCAACG13400.034.5542953
CAACGCA13600.034.0461435
TCAACGC13700.033.797634
AACGCAG13750.033.674736
AGAGTAC14500.031.45632211
CAGAGTA14950.030.74060810
CGCAGAG15300.030.2632378
ACGCAGA15450.029.969427
GCAGAGT16200.028.7952429
TCTGGAG11400.028.66731570
TGCTTTA3100.027.86636514
ATTAGTC500.007674401627.6434338
GTATAAG2050.026.9810661
TAATGTG658.575645E-426.5802255
Produced by FastQC (version 0.11.5)