FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796163
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAACA26890.33774490901988663No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20570.2583641791944614No Hit
GTATATTACTGTGTGAAGGCCGCGGGCCACGGTGACAACCACGAGTCTCT18720.23512773138163917No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18190.22847080308931716No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16020.20121507781697967No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15450.19405573984221824No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14310.17973706389269534No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13820.1735825452828127No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13360.1678048339347596No Hit
CTCCTGTGCAGTGTCTGGATTCACCTTCAGTACCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG13200.165795195205002No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGTGACAGAATTCA13180.16554399036378228No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13170.16541838794317243No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12600.15825904996841098No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12210.15336055556462683No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12160.15273254346157758No Hit
ATACTACACAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATT11690.14682922969291465No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11200.14067471108303198No Hit
GCACTGAACACAGAGGACTCAGCATGGAGTTTGGGCTGAACTGGGTTCTC10920.1371578433059562No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10640.13364097552888038No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10430.13100332469607354No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10380.1303753125930243No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10080.1266072399747288No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10080.1266072399747288No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9890.12422079398314165No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9680.12158314315033479No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9670.12145754072972494No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9660.12133193830911508No Hit
CTACACAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATTTCAAGAACACACTGTTTCTGCAAATGAA9620.12082952862667569No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9170.11517741969923245No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8810.11065573255727784No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8800.110530130136668No Hit
CAGTAATATACAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAA8770.11015332287483844No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8750.10990211803361875No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8490.10663645509776264No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8450.10613404541532324No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGGTAC7970.10010512922605044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.041.875351
GTGGTAT1900.038.115641
TATCAAC14550.036.7233732
TATAGTG501.8696192E-434.4725845
TCAACGC15450.034.13794
CAACGCA15500.034.0277825
ATCAACG15750.033.2687843
AACGCAG15800.033.1635066
TTCGGGG9150.031.59528770
TATAAGA1900.030.8438932
AGAGTAC17400.030.5236411
GTATAAG2050.030.2800241
CAGAGTA17750.029.92176410
GCAGAGT17650.029.7004999
CGCAGAG17800.029.6421038
AAACACA4550.029.00637670
ACGCAGA18200.028.7939077
GTGTATA1103.6750862E-828.2154751
TAGTCAT500.007276437727.94664258
TCTGGAG11450.026.89528870
Produced by FastQC (version 0.11.5)