FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453602
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62110.4272833966931801No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC43040.2960920527076875No Hit
GTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT42490.29230834850254744No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAA40500.2786182187421316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38520.2649968836036274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36260.24944929905159735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36100.24834858510101115No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34970.2405747928249961No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32500.22358252121282168No Hit
GGTCTAGTCAGAGCCTCGAATACAGCGACGGAAACATCCACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTC32110.2208995309582678No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31420.2161527020463648No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30710.21126828389063856No Hit
GGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT29820.20514556254050284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27160.18684619311200729No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG26840.1846447652108349No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTCAA25440.17501351814320562No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25050.17233052788865177No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24960.17171137629144706No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC23650.16269928082102253No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTGAGTGTGAAATCAGTGCCTGACCCACTGCCG23170.15939713896926394No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22480.15465031005736096No Hit
AGGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT22360.15382477459442132No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22140.15231129291236528No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAAGTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA21870.15045383812075108No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAATA21260.1462573661841412No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20250.1393091093710658No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20160.13868995777386106No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGAC20140.1385523685300378No Hit
GTCTATTACTGTGCGACTCTTTCCGGGGTGGCAGTTATTTTCCCCTACCAGTACGACGGTTTGGACGTCTGGGGC19830.13641973525077702No Hit
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTCTTCATTTGCAGATACA19430.13366795037431156No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA19430.13366795037431156No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19380.13332397726475337No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19270.13256723642372534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18390.12651330969550126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18200.12520621187918013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17710.1218352754055099No Hit
CTTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACA17680.12162889153977498No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17540.12066576683301207No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC17190.11825795506610476No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT16900.11626291103066727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16630.11440545623905307No Hit
GTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGACTCTGGGGTCC16260.11186005522832247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16050.11041536816817808No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACAC15880.10924585959568026No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15580.10718202093833112No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15440.10621889623156819No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACTCTCC15220.10470541454951218No Hit
TTATTACTGCATGCAAGGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA15210.10463661992760054No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15170.10436144143995399No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15020.10332952211127944No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC14970.10298554900172124No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14790.10174724580731176No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14690.1010592995881954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.042.33411
GTATCAA33500.041.7949181
TGGTATC4850.038.0064352
TATCAAC37050.037.6825452
ATCAACG37900.036.8361553
TCAACGC38300.036.362324
CAACGCA39100.035.5310335
AACGCAG39950.034.8604976
GTGGTAT5350.034.4686621
ACGACGG5650.032.9560853
ACGTCTG5950.032.40808565
AGAGTAC42300.032.30713311
ACGCAGA43150.032.2807967
CGACGGT5800.032.12765554
CGCAGAG43450.032.0722588
ACGGTTT6000.031.72025156
CAGTACG5850.031.12743849
GTACGAC6000.030.9941451
TACGACG6050.030.75647752
CAGAGTA44850.030.62262210
Produced by FastQC (version 0.11.5)