FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104145
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGAAGATAGA53880.48797938676532515No Hit
GTGTATTACTGTGCAAGAGGACCCGAGGGATATTGTAGTGGTAGTAGCTGCTATTCCTACTTTTACTTCGATCT35600.3224214210995838No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28790.2607447391420511No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28390.2571220265454266No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26440.23946130263688192No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23600.2137400432008477No Hit
GTATTACTGTCAGCAGTATGGTAACTCACCGCTCACTTTCGGCGGAGGGA22330.2022379307065648No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21350.19336228484483467No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21170.19173206417635366No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21040.19055468258245067No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20080.1818601723505518No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCC18890.17108260237559378No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18590.1683655679281254No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18030.16329377029285103No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAAGTACGACATGCACTGGGTCCGCCAAACTACAGGAAAACGT17910.16220695651386366No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17890.16202582088403245No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16650.15079541183449638No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16370.1482595130168592No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16230.1469915636080406No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15890.14391225790090975No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15820.14327828319650046No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15710.14228203723242872No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15020.13603285800325138No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14340.12987424658898966No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAGCAGCTACTTGGGCTGGTACC13960.12643266962219638No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG13900.12588926273270268No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13770.12471188113879972No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT13700.12407790643439041No Hit
CAGTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGAAGATA13680.12389677080455919No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13460.1219042788764157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13320.12063632946759709No Hit
GTATGGTAACTCACCGCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT13270.12018349039301904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13150.11909667661403167No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC12970.11746645594555064No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC12950.1172853203157194No Hit
ATCCAGGCTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAAATGCCAAG12950.1172853203157194No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12940.11719475250080379No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTTTATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT12850.11637964216656327No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12760.11556453183232274No Hit
CTACAATATCCCTCGGGTCCTCTTGCACAGTAATACACAGCCGTGTCCCC12610.11420601460858855No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12600.11411544679367294No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTAAAAGTAGGAATAGCAGCTACTACCACTACAATATCCCTCGGGTCCT12270.11112670890145769No Hit
GGATAGTATGTGTCACCAGCAGTAGCAATAGATGAGACCCACTCCAGACG12260.11103614108654207No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12250.11094557327162646No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAG12140.10994932730755472No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12110.10967762386280787No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA11990.10859081008382052No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11970.1084096744539893No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11760.1065077503407614No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG11730.10623604689601457No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11700.10596434345126772No Hit
CTCTTGCACAGTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATT11570.10478696185736475No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11450.1037001480783774No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11270.10206992740989634No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA11260.10197935959498072No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAAGTACGACATGCACTGGGTCCG11240.1017982239651495No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11090.10043970674141531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)