FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173226
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA88710.7561203041869171No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT69400.5915313844050507No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54820.46725865263811067No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36420.3104261242079531No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35520.30275496792604323No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34970.2980670390870983No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34950.29789656894750033No Hit
GTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTCAGGAG33870.28869118140920846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33810.2881797709904145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32900.28042337963870556No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT32000.2727522233567957No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA31990.2726669882869967No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA29560.25195486632584No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACGTTCAGGGAGA28810.24556223609091513No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG28760.24513606074192013No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC28350.24164142288016122No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27230.23209509506267337No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26160.2229749425941805No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25730.21930983459282355No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA25620.21837224882503453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24120.20558698835518477No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23730.20226282063302384No Hit
GGCCAAAAGTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCA22960.19569972025850094No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG22950.19561448518870192No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22940.1955292501189029No Hit
GTCTCTGGTGGCTCCATCAGAAGTGCTAATTATTACTGGGGCTGGATCCG22090.18828426918598803No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21720.18513057160342508No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA21380.18223257923025912No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC21180.18052787783427915No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20400.17387954238995726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20310.17311242676176627No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGA20240.17251578127317327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.1711520201563893No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20060.1709815500167913No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19970.17021443438860032No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19540.16654932638724337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19520.1663788562476454No Hit
GGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGCCGG19490.1661231510382484No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18810.16032716629191648No Hit
GTCCCTGACCCGCTGCCACTGAACCGATGTGGCACTCCAGAGAATTGGTT18770.15998622601272047No Hit
ATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA18760.15990099094292148No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCT18720.1595600506637255No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18550.15811105447714252No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT18530.15794058433754452No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18470.15742917391875053No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGA17700.15086607354422762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17590.14992848777643863No Hit
GATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTG17450.14873519679925268No Hit
GTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTGGCACTCCA17430.14856472665965464No Hit
GTGTATTACTGTGCGAGACGGGGGTATTGTGGTGGAGACTGCTGGGATGC17100.1457519693562877No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17070.1454962641468907No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17040.1452405589374937No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGT16980.14472914851869972No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16750.14276874191332276No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16490.1405526300985488No Hit
CCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTG16440.1401264547495538No Hit
CTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGAT16420.13995598460995579No Hit
GTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCT16190.13799557800457884No Hit
GTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATA16140.13756940265558382No Hit
AGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTC15990.13629087660859887No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCC15610.1330519439562369No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15570.13271100367704092No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15320.13058012693206594No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14720.12546602274412602No Hit
TCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTGGCACT14700.12529555260452802No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14630.12469890711593504No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14470.12333514599915105No Hit
TTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGA14430.12299420571995506No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG14260.12154520953337208No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGACAATCAGTGCCG14200.12103379911457811No Hit
GTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATG13890.11839151195080913No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13840.11796533660181414No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13760.11728345604342216No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACAC13610.11600492999643718No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13560.11557875464744219No Hit
GGTCAGGGACAGATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGAT13430.11447069874005521No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13410.1143002286004572No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13390.11412975846085921No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13180.11233982199508025No Hit
GATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAAT13130.11191364664608523No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13120.11182841157628624No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13120.11182841157628624No Hit
GATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTT12960.11046465045950225No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAA12930.11020894525010526No Hit
CTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTAT12920.11012371018030627No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGAAGTGCTAATTATTACTGGG12750.10867471399372329No Hit
GTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12710.10833377371452729No Hit
GTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA12700.1082485386447283No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGACCTCACGTTCAGGGA12700.1082485386447283No Hit
ATTCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGATAGTGGAGT12610.10748142301653729No Hit
GATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGC12590.10731095287693931No Hit
GGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAG12580.10722571780714032No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12570.10714048273734131No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12560.1070552476675423No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12500.10654383724874833No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12470.10628813203935132No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12470.10628813203935132No Hit
GTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTC12390.10560625148095934No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12250.10441296050377337No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12070.10287872924739137No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11960.10194114347960238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)