FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721072
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT12980.18000976324139614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28200.041.7418021
CGTATGC3250.037.4125241
TCGTATG3250.037.3652840
TATCAAC32100.036.7517742
ACGCGTA3200.036.49361827
GGTATCA10450.035.467751
CTCGTAT3150.035.2174239
GTGGTAT4950.034.6649861
ATCAACG34900.033.9014783
AGGGGGG4550.033.87820470
CAACGCA35500.033.038685
AACGCAG35700.032.9496546
TATACAC3750.032.9227523
TCAACGC36350.032.5491454
TAGTAAT3400.032.4188432
GACGCGT3650.031.98996226
TATGCCG3700.031.97284143
TGGTATC5150.031.9638372
GTAGTAA3800.031.7124231
CTTATAC4000.030.8865011
Produced by FastQC (version 0.11.5)