FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601942
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11640.19337411245601735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2550.068.3836770
CTTATAC1900.055.976271
GAGTATC200.00692443152.54965252
TTATACA2050.051.8502772
TATACAC2050.051.8502773
TCTTATA200.00752144251.462061
TACACAT2300.049.1959535
ATACACA2250.048.765284
GTATCAA30650.039.7368771
CTCGTAT2600.038.6781639
AAATCTC2700.038.41057235
CAGAAAT2700.038.3239132
CTTGAAA2900.037.59916357
ACACATC3050.037.0985876
GGTATCA11900.036.9027631
GTGGTAT4750.036.8359951
TATCAAC33600.036.2269972
TTGAAAA3050.035.78967358
AAAGGGG3000.035.5204668
TGGTATC5250.035.2677462
Produced by FastQC (version 0.11.5)