FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601942
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23660.39306112549049577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAG250.001357738779.2650870
TATCAGT150.002409884768.6002662
GCTTAGT309.412372E-657.1953931
CATTAGC200.007180833352.06872640
TATACCC200.007528298551.4502034
CTTAGTT306.492281E-445.733512
GGTATCA14800.037.331591
GTATCAA33000.036.085091
TTGTATC601.3919715E-534.31438417
GTGGTAT6050.034.0336231
TATCAAC35500.033.8170362
TGGTATC6650.030.9474892
AACGCAG39650.030.0180236
ATCAACG41350.028.8668583
CAACGCA41350.028.7009565
CAGAGTA39750.028.58799610
AGAGTAC39700.028.53752311
GCAGAGT41000.028.3025579
ACGCAGA42350.028.0325647
TCAACGC42950.027.7914924
Produced by FastQC (version 0.11.5)