FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747683
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27350.3657967347124383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38650.040.502351
GGTATCA15600.037.410131
GTGGTAT5850.036.970011
TATCAAC42950.036.4254652
TGGTATC6500.033.7808072
CAACGCA47850.032.8387765
AACGCAG48050.032.5614626
ATCAACG47950.032.5556373
AGAGTAC46500.032.26215411
CAGAGTA46700.032.197510
TCAACGC48700.032.1951684
GCAGAGT49100.031.6703269
ACGCAGA50000.031.2978467
CGCAGAG49850.031.1896698
AGAGTCG2400.028.60774611
ATAGTGC500.0079433827.4487446
GAGTACG52950.026.45203612
AGTACGG52900.026.41214413
ATTGTTT1000.00958779226.40674470
GCCGTTA809.885178E-525.74697115
Produced by FastQC (version 0.11.5)