FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988786
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22370.22623702196430773No Hit
AGTCAGGACATTGGCCTTTATTTAGCCTGGATTCAGCAGAAGCCAGGGAAAGCCCCTAAGTCCCTCATCTATG20250.20479658894846814No Hit
GTATGATAGGTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGACATCAAGCGAACTGTGGCTGCACCAT19060.19276162890655815No Hit
CATCTATGCTGCATCCAGTTTGCGAGATGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAAATTTCA17310.17506315825669053No Hit
ATCATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC15440.156151078190832No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15200.15372385935885016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14230.14391384991292352No Hit
CAGTATGATAGGTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGACATCAAGCGAACTGTGGCTGCACCAT13510.136632193416978No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13390.13541858400098705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.13218229222501127No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13030.1317777557530143No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12670.12813692750504152No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC12590.1273278545610476No Hit
CTATCATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTC12490.12631651338105515No Hit
GTGCAGCCACAGTTCGCTTGATGTCCAGCTTGGTCCCCTGGCCAAAATTG12300.12439496513906953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11790.11923712512110811No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11390.11519176040113836No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10570.1068987627252004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.042.4682541
TCGTATG1100.040.90754340
GGTATCA8950.038.7552951
TATCAAC25100.037.734492
ATCAACG25650.036.791583
CAACGCA26400.035.616385
TCAACGC26500.035.6114734
AACGCAG27200.034.6950046
AGAGTAC29950.031.39473711
ACGCAGA30650.030.677737
CAGAGTA31000.030.55276510
CGCAGAG31300.030.150298
TATGCCG1401.2732926E-1129.75115643
GCAGAGT31750.029.722969
CGTATGC1551.8189894E-1229.05042541
GTCGGAT850.00644765128.64598370
CGATTGT1504.428226E-628.40726770
CTCGTAT1254.0654413E-927.6531839
GCTAATC1503.6379788E-1127.55213233
AGTACGG21950.027.04672613
Produced by FastQC (version 0.11.5)