FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988786
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21280.21521340310239023No Hit
GTATGATAGGTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGACATCAAGCGAACTGTGGCTGCACCAT20290.20520112542046512No Hit
AGTCAGGACATTGGCCTTTATTTAGCCTGGATTCAGCAGAAGCCAGGGAAAGCCCCTAAGTCCCTCATCTATG17860.18062553474664894No Hit
CATCTATGCTGCATCCAGTTTGCGAGATGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAAATTTCA16620.16808490411474272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16280.16464634410276846No Hit
ATCATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC15830.16009530879280248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14010.14168889931694015No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13190.1333959016410022No Hit
CAGTATGATAGGTACCCGTACAATTTTGGCCAGGGGACCAAGCTGGACATCAAGCGAACTGTGGCTGCACCAT12890.1303618781010249No Hit
CTATCATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTC12590.1273278545610476No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12360.125001769847065No Hit
GTGCAGCCACAGTTCGCTTGATGTCCAGCTTGGTCCCCTGGCCAAAATTG12310.12449609925706878No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12220.12358589219507558No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11620.11751784511512098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11450.11579856510913382No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC11320.11448382157514367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10390.10507834860121401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.038.3453641
GTATCAA23550.037.482111
TATCAAC25100.035.015232
ATCAACG26500.033.0363163
TCAACGC26550.032.8452954
CAACGCA27200.032.060395
AACGCAG27950.031.3224456
TAATCGT802.7134156E-630.25503238
GTGGTAT4750.030.251781
TGGTATC4550.030.0639552
GCTATAC704.0887084E-529.3123553
AGAGTAC30600.028.40360511
CAGAGTA31150.028.01194610
ACGCAGA31800.027.6419967
GCAGAGT32350.027.2901929
CGCAGAG32350.027.1802888
AATAGCG807.9236874E-526.59929562
TTCGTAT1209.503958E-426.11447570
AACGAAC1001.3829873E-524.53520851
AGTACGG23200.024.04145613
Produced by FastQC (version 0.11.5)