FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427965
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10820.2528244132113608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTTA200.007526754451.45031410
AGGGGGG2600.050.46151770
CTTATAC2400.047.16831
TTATACA2750.042.407532
GGTATCA5950.041.5109861
TATACAC2750.041.160253
CTCGTAT2400.040.4183139
TACACAT2900.039.0312735
TCGTATG2950.038.78206340
GTATCAA13500.038.3699041
CGTATGC3050.037.5459941
GTGGTAT2100.035.937751
ATACACA3150.035.933554
TCTACAT3300.033.3738932
ACGAGAC3400.033.29916422
TATCAAC15800.032.997672
TCTCGTA2850.032.79379338
TATGCCG3550.032.31894743
CCGTCTT3700.032.06609747
TATCATA553.1095633E-431.6066843
Produced by FastQC (version 0.11.5)