FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427965
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20560.48041311789515495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC200.00753937451.4285121
GTAACGA305.4308714E-447.41679865
TGGTATC4050.043.974822
GTGGTAT3900.043.031571
TTGTACG350.001395966639.1424263
GTATCAA16050.033.289351
GGTATCA8000.032.109021
GTCTAGC605.6524185E-428.541351
TATCAAC18750.028.4956862
AACGCAG19250.027.9334556
ACTATGC500.00799098227.41250819
CAACGCA19850.027.0891195
ACGCAGA20100.026.7553187
CAGAGTA19950.026.62242110
TCAACGC20300.026.4886234
CGCAGAG20650.026.2147088
ATCAACG20500.026.0631263
GTAGTTT1250.001043507425.69171570
AGAGTAC20700.025.65784311
GCAGAGT21450.025.2399549
Produced by FastQC (version 0.11.5)