FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325176
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC59880.4518645070541573No Hit
GCCTTGGGCTGACCTAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCA56700.4278676945552892No Hit
GACCTAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCAAGTAGTTAGA48100.36297065446401083No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44310.3343706798191335No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40540.3059216285233056No Hit
ATAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG40280.30395962498566226No Hit
GATTACTATTGTGCAACATTGGATGACAGTCTAACTACTTGGGTGTTCGG38540.2908292936183571No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCTCCCTCAGCGTCTGGGACCC37730.284716897981853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34520.26049370045941067No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34050.2569470017567478No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33310.2513628378419168No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGTTTGGTCCCTC32090.24215651354989828No Hit
GTATTACTGTGCAAAGTCAGTGGGAACTATTACGTCGGACACACGAGACT31290.2361195795879189No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31000.23393119102670135No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCTCCCTCAGCGTCT30410.22947895222974157No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28160.21250007546167451No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27700.2090288384335364No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27140.2048029846601508No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26190.19763412558030027No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25990.19612489208980544No Hit
CAGTAATACAGGGCGGTGTCCTCAACTCTCAGACTGTTCATTTGTAGATA24950.18827687793923223No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA24750.18676764444873736No Hit
GTGCTGACTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC24690.18631487440158892No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23900.18035340211413425No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23710.17891963029816418No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT23500.17733493513314458No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAATTGCTGGTACCAATATA23420.17673124173694663No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22430.16926053595899715No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22340.16858138088827446No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22130.16699668572325488No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21940.16556291390728478No Hit
GCCCCCAAACTCCTCATCTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT21850.1648837588365621No Hit
GTAATACAGGGCGGTGTCCTCAACTCTCAGACTGTTCATTTGTAGATATA21510.16231806190272086No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21300.16073336673770128No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCTC21290.1606579050631765No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21220.16012967334150333No Hit
ACATTGGATGACAGTCTAACTACTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTAGGTCAGCCCAAGG20950.15809220812933528No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20560.1551492028228703No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20520.15484735612477135No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20130.15190435081830642No Hit
CTGTATTACTGTGCAAAGTCAGTGGGAACTATTACGTCGGACACACGAGACTATTACTACTACGGTATGGACGT19890.1500932706297126No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG19410.14647111025252493No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19240.14518826178560432No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19070.14390541331868373No Hit
GTTTGGGGGCCGTGCCTGGGAATTGCTGGTACCAATATACATAATTAATG19050.14375448996963422No Hit
GATTAGTCCCATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAATTGCTGG18970.14315079657343627No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTATTGTGCAA18900.14262256485176308No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18890.14254710317723834No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18780.1417170247574662No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG18770.14164156308294143No Hit
GTCTAACTACTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18690.1410378696867435No Hit
AGACTATCCTGTGCAGCCTCTGGATTCATCTTTGATGATTATGCCCTTCA18410.13892494280005072No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18170.1371138626114569No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18100.1365856308897837No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17940.13537824409738783No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17940.13537824409738783No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17920.13522732074833832No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17860.13477455070118988No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17730.13379354893236822No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17610.13288800883807134No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17580.1326616238144971No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17540.13235977711639813No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17370.1310769286494775No Hit
CCCAAGTAGTTAGACTGTCATCCAATGTTGCACAATAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGG17300.1305486969278043No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17270.13032231190423008No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16830.1270019982251414No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16720.12617191980536924No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG16540.1248136096639239No Hit
CTCCAGACCCTTCCCCGGACCTTGACGGACCCAATGAAGGGCATAATCAT16330.1232289144989043No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16230.12247429775365687No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15930.12021044751791461No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15880.1198331391452909No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15700.11847482900384552No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15570.11749382723502387No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGT15520.11711651886240017No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15440.11651282546620223No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15350.11583367039547955No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG15320.11560728537190532No Hit
ATCCAATGTTGCACAATAGTAATCAGCCTCATCCTCGGACCGGAGCCCAC15300.11545636202285584No Hit
GTTTGGTCCCTCCGCCGAACACCCAAGTAGTTAGACTGTCATCCAATGTTGCACAATAGTAATCAGCCTCATCCT14960.11289066508901459No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14800.11168327829661873No Hit
CTACTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTAGGTCAGCCC14790.11160781662209397No Hit
GTCCGAGGATGAGGCTGATTACTATTGTGCAACATTGGATGACAGTCTAACTACTTGGGTGTTCGGCGGAGGGAC14790.11160781662209397No Hit
GTCCCATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAATTGCTGGTACCA14760.11138143159851975No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14650.11055135317874759No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT14590.11009858313159912No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14480.10926850471182695No Hit
CTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14470.10919304303730222No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14440.10896665801372798No Hit
ATCCTGTGCAGCCTCTGGATTCATCTTTGATGATTATGCCCTTCATTGGGTCCGTCAAGGTCCGGGGAAGGGT14380.10851388796657953No Hit
GATCAGCACTGAACACAGAACTCACCATGGAGTTTGGACTGAGCTGGGTC14320.10806111791943109No Hit
GCACTGAACACAGAACTCACCATGGAGTTTGGACTGAGCTGGGTCTTCCT14180.10700465447608469No Hit
GGATAGTCTCAGGGACCCCCCAGGCCGAACCACGGCTCCCCCAGATTCCACCAGTTGTACCTCACACTGGACAC14120.10655188442893623No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13890.10481626591486717No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13830.1043634958677187No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13700.10338249409889706No Hit
GAGTTTGGACTGAGCTGGGTCTTCCTTGTTGCCATTTTAAAAGGTGTCCAGTGTGAGGTACAACTGGTGGAAT13610.10270333902817437No Hit
GTCATCCAATGTTGCACAATAGTAATCAGCCTCATCCTCGGACCGGAGCC13470.10164687558482798No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13430.101345028886729No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13400.10111864386315478No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG13370.10089225883958056No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13330.1005904121414816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1950.049.4995161
GGTATCA6550.047.3673131
TGGTATC2300.041.9495622
GTATCAA15850.039.8014951
TCGTATG350.001321520839.58735740
CTCGTAT350.00132670439.5558539
TATCAAC17650.035.727582
TGTAATA807.171002E-834.4585722
ATCAACG19050.033.1019333
CAACGCA19250.032.758025
TCAACGC19250.032.758024
AACGCAG19800.031.8480726
CGCAGAG21250.029.673798
TTCGGGG13200.029.62068470
AGAGTAC21400.029.30588711
CAGAGTA21950.028.5715710
GCAGAGT22350.028.2133339
GTATTAA500.007765078527.5783021
GTACTTT6100.027.11596314
GTCCTAA1155.6837962E-826.9787731
Produced by FastQC (version 0.11.5)