FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325176
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54720.41292628299939027No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCTCCCTCAGCGTCTGGGACCC47060.3551226403134376No Hit
GCCTTGGGCTGACCTAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCA42620.321617656824452No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40730.3073554003392757No Hit
GATTACTATTGTGCAACATTGGATGACAGTCTAACTACTTGGGTGTTCGG38660.291734833712654No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38590.2912066019909808No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCTCCCTCAGCGTCT37790.28516966802900146No Hit
GACCTAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCAAGTAGTTAGA36100.27241664503432No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33750.2546831515210055No Hit
GTGCTGACTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC33110.249853604351422No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32780.2473633690921055No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31060.23438396107384984No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30130.22736602534304878No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28960.2185370094236539No Hit
GTATTACTGTGCAAAGTCAGTGGGAACTATTACGTCGGACACACGAGACT28530.21529215741909No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27690.20895337675901163No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26150.19733227888220128No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25270.19069165152402398No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA25190.19008795812782603No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCTC24480.18473017923656931No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGTTTGGTCCCTC24170.18239086732630233No Hit
GCCCCCAAACTCCTCATCTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT23790.1795233236943621No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT23790.1795233236943621No Hit
CAGTAATACAGGGCGGTGTCCTCAACTCTCAGACTGTTCATTTGTAGATA23610.17816501355291675No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA23030.1737882364304817No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22760.17175077121831364No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAATTGCTGGTACCAATATA22610.1706188461004425No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22380.16888322758637345No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22180.16737399409587858No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21660.163449987020592No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21120.1593750565962559No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20740.1565075129643157No Hit
GTAATACAGGGCGGTGTCCTCAACTCTCAGACTGTTCATTTGTAGATATA20710.15628112794074145No Hit
GTTTGGGGGCCGTGCCTGGGAATTGCTGGTACCAATATACATAATTAATG20400.15394181603047444No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20180.15228165919093012No Hit
ACATTGGATGACAGTCTAACTACTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTAGGTCAGCCCAAGG20040.15122519574758372No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG19960.15062150235138577No Hit
AGACTATCCTGTGCAGCCTCTGGATTCATCTTTGATGATTATGCCCTTCA19920.1503196556532868No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19840.14971596225708889No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTATTGTGCAA19220.14503733843655486No Hit
CTGTATTACTGTGCAAAGTCAGTGGGAACTATTACGTCGGACACACGAGACTATTACTACTACGGTATGGACGT19210.1449618767620301No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19070.14390541331868373No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG18680.14096240801221874No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18550.1399814062433971No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18550.1399814062433971No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18480.1394531745217239No Hit
GATTAGTCCCATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAATTGCTGG18360.13854763442742699No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18160.13703840093693215No Hit
GTCTAACTACTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17870.13485001237571462No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17540.13235977711639813No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG17440.1316051603711507No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17430.13152969869662598No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17400.13130331367305173No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17210.12986954185708163No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17000.12828484669206203No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17000.12828484669206203No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16720.12617191980536924No Hit
ATAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG16640.1255682264091713No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16540.1248136096639239No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16460.12420991626772594No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15960.12043683254148883No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15930.12021044751791461No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15910.12005952416886512No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15900.11998406249434036No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15760.11892759905099398No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15650.11809752063122181No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15560.11741836556049914No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15490.11689013383882595No Hit
GTCCCATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAATTGCTGGTACCA15430.11643736379167748No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15410.116286440442628No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG15380.11606005541905377No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15290.11538090034833108No Hit
CTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC15020.11334343513616306No Hit
CTACTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTAGGTCAGCCC14990.11311705011258881No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14970.11296612676353933No Hit
ATCCTGTGCAGCCTCTGGATTCATCTTTGATGATTATGCCCTTCATTGGGTCCGTCAAGGTCCGGGGAAGGGT14950.11281520341448983No Hit
CTCCAGACCCTTCCCCGGACCTTGACGGACCCAATGAAGGGCATAATCAT14890.11236243336734139No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14680.11077773820232181No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14600.11017404480612386No Hit
GTCCGAGGATGAGGCTGATTACTATTGTGCAACATTGGATGACAGTCTAACTACTTGGGTGTTCGGCGGAGGGAC14570.10994765978254964No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14550.10979673643350014No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14400.10866481131562902No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14330.10813657959395581No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13990.10557088266011458No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13940.10519357428749086No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13870.10466534256581767No Hit
GGATAGTCTCAGGGACCCCCCAGGCCGAACCACGGCTCCCCCAGATTCCACCAGTTGTACCTCACACTGGACAC13770.10391072582057026No Hit
GATCAGCACTGAACACAGAACTCACCATGGAGTTTGGACTGAGCTGGGTC13700.10338249409889706No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13680.10323157074984757No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13610.10270333902817437No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13570.1024014923300754No Hit
GTCTGTGCTGACTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG13550.10225056898102591No Hit
GAGTTTGGACTGAGCTGGGTCTTCCTTGTTGCCATTTTAAAAGGTGTCCAGTGTGAGGTACAACTGGTGGAAT13370.10089225883958056No Hit
AATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG13300.10036402711790736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)