FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049759
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14930.14222311978273108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21400.040.8124851
GGTATCA8950.039.4167481
TATCAAC23500.037.144172
TCAACGC24900.034.7791374
ATCAACG25200.034.500933
CAACGCA25500.033.9608045
AACGCAG25900.033.5684746
CGCAGAG29200.030.0263848
CAGAGTA30650.028.71762710
AGAGTAC31300.028.45087411
ACGCAGA31150.028.0274227
GCAGAGT31650.027.810289
GTGTATA1950.026.346521
AGTACGG24850.025.91182713
TATATCG3300.023.8567625
TACGGGG15200.023.20920615
GAGTACG28150.023.11754812
GTACGGG28850.022.20048114
GTGGTAT4650.022.0970821
GTACGGA953.2195245E-421.631881
Produced by FastQC (version 0.11.5)