FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963659
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC25120.2606731219238341No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22840.23701330034794468No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20660.214391190244682No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20250.2101365732069124No Hit
CTATAATAGTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18660.19363696079214743No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18430.19125022440510595No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16390.17008091036352072No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15590.1617792185825069No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15010.15576049204127188No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14940.15503409401043314No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14890.15451523827411978No Hit
GATCTATAGGGCGTCTACTTTAGAAGTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA14240.14777011370204607No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG13490.13998727765734562No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13490.13998727765734562No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13240.13739299897577878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13020.13511003373599997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12940.1342798645578986No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12850.13334592423253452No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12830.1331383819380092No Hit
GAGTATAAGTACTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGT12760.1324119839071705No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12700.13178935702359446No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12660.13137427243454375No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12420.1288837649002396No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12290.12753473998582485No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATAGTTAT11750.12193109803364052No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11700.12141224229732717No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11630.12068584426648847No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11550.11985567508838708No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11480.11912927705754836No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11420.11850665017397234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11310.11736516755408291No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11290.11715762525955759No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10950.11362940625262671No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC10770.11176152560189859No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10760.11165775445463592No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10580.10978987380390781No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10270.10657296823876494No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT10250.10636542594423962No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10230.10615788364971426No Hit
GGAATATATGGCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATACA10230.10615788364971426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10230.10615788364971426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10220.10605411250245159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10190.10574279906066357No Hit
GTCTGCATCTGTCGGAGACAGAGTCACTCTCACTTGCCGGGCCAGTCAGA10160.10543148561887555No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10080.10460131644077417No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9910.10283720693730874No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9870.10242212234825804No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9840.10211080890647002No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9740.1010730974338433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14850.039.1717721
GTGGTAT2450.036.5275271
TATCAAC16150.036.2185942
ATCAACG17950.032.5849533
AACGCAG18200.032.32646
TCAACGC18350.032.0621574
CAACGCA18450.031.8883745
ACCGTTC450.00467262930.5988798
AGAGTAC19650.029.43709411
GCAGAGT20750.028.374379
CGCAGAG20900.028.0001748
CGAACTA756.2075596E-527.5704829
GTGTAAC500.00782223427.5361351
ATAGGAC500.00783809827.5247023
TACGGGT1800.026.7809715
ATTAGAG658.795258E-426.466063
TTCGGGG9550.025.80722870
TATTAGA700.001354679724.5769022
GTATTAG1001.5916168E-524.0941181
AGTACGG20200.023.52209513
Produced by FastQC (version 0.11.5)