FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616756
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33410.541705309717295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCA205.5732246E-499.1792570
ATTGTGC200.007164130552.09956741
GCTTATA200.00745378351.5799671
GTATCAA35300.040.621051
GGTATCA13900.040.5712971
TAACCTG350.001322466939.5782235
GTGGTAT4900.037.1937181
TATCAAC38800.037.03942
TGGTATC5100.036.403492
AACGCAG43450.032.9172026
ATCAACG44150.032.4731753
CAGAGTA42650.032.1018410
AGAGTAC42550.032.0964411
GCAGAGT44250.031.8739959
ACGCAGA45050.031.7532627
CAACGCA45050.031.7481145
TGAGTAC553.3218763E-431.255524
TCAACGC45650.031.2555184
CGCAGAG45700.031.0081968
CAGAGTC1401.4551915E-1129.48622310
Produced by FastQC (version 0.11.5)