FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497387
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9970.2004475388379672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA308.9177265E-657.71621338
AGGGGGG3150.055.09946470
TATACTG200.007577057551.3649335
TATACAC2450.043.328243
CTTATAC2450.041.9600871
GTGGTAT2950.041.817851
GTATCAA19800.040.3247761
TTATACA2550.040.2902762
CTCGTAT2050.038.88844739
ATCGTAT459.735915E-538.51271439
ATATCGT459.841431E-538.44230337
TCGTATG2550.038.1061640
GGTATCA7850.037.9778861
CGTATGC2800.035.979941
ATACACA2900.035.424094
TATCAAC22700.034.85022
GTATGCC3100.034.7569442
TGGTATC3600.033.2954372
ACACATC3250.032.662836
ATCAACG24400.032.4188543
Produced by FastQC (version 0.11.5)